Analyses on a molecular mechanism of spontaneous articular cartilage regeneration induced in vivo by double-network gel

双网络凝胶体内诱导关节软骨自发再生的分子机制分析

• 批准号: 20240045
• 负责人: YASUDA Kazunori
• 金额: $ 32.36万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-20240045/
• 关键词: 関節軟骨; 自然再生; 再生誘導材料; ダブルネットワークゲル; 分子機序; 関節軟骨欠損; 硝子軟骨; 高機能生体材料; 再生誘導

(1) We clarified genetic characteristics of the articular cartilage spontaneously regenerated by double-network (DN) gel, using microarray. (2) We found that PAMPS, a component of the DN gel, has an ability that can induces the spontaneous articular cartilage regeneration. (3) We observed marker genes in a chondrogenic differentiation process, using multi-colored bioluminescence probes. We discovered a synchronized oscillation phenomenon of cell metabolism in prechondrogenic condensation. (4) We developed the transgenic bioluminescence mouse to be used for in vivo imaging. (5) These results provided important information to clarify a comprehensive mechanism of the spontaneous cartilage regeneration phenomenon.

(1)利用微阵列技术明确了双网凝胶（DN）自发再生关节软骨的遗传特征。(2)我们发现DN凝胶成分PAMPS具有诱导关节软骨自发再生的能力。(3)我们使用多色生物发光探针观察了软骨分化过程中的标记基因。我们发现了在软骨形成前凝结过程中细胞代谢的同步振荡现象。(4)研制了用于体内成像的转基因生物发光小鼠。(5)这些结果为阐明软骨自发再生现象的综合机制提供了重要信息。

项目成果

Implantation of Autogenous Meniscal Fragments Wrapped With a Fascia Sheath Enhances Fibrocartilage Regeneration In Vivo in a Large Harvest Site Defect

• DOI: 10.1177/0363546509350749
• 发表时间: 2010-04-01
• 期刊: AMERICAN JOURNAL OF SPORTS MEDICINE
• 影响因子: 4.8
• 作者: Kobayashi, Yasukazu;Yasuda, Kazunori;Tohyama, Harukazu
• 通讯作者: Tohyama, Harukazu

Implantation of comminuted autogenous meniscus tissue into a large meniscus defect enhances spontaneous in situ regeneration of the meniscus an experimental study

将粉碎的自体半月板组织植入到大的半月板缺损中可增强半月板的自发原位再生实验研究

• 发表时间: 2009
• 作者: Kobayashi Y;Yasuda K;Kondo E;Katsura T;Kumura M
• 通讯作者: Kumura M

Bioluminescence imaging of dual gene expression at the single-cell level

• DOI: 10.2144/000113419
• 发表时间: 2010-06-01
• 期刊: BIOTECHNIQUES
• 影响因子: 2.7
• 作者: Kwon, HyuckJoon;Enomoto, Toshiteru;Ohmiya, Yoshihiro
• 通讯作者: Ohmiya, Yoshihiro

Ultrathin tough double network hydrogels showing adjustable muscle-like isometric force generation triggered by solvent

• DOI: 10.1039/b916581a
• 发表时间: 2009-01-01
• 期刊: CHEMICAL COMMUNICATIONS
• 影响因子: 4.9
• 作者: Liang, Songmiao;Yu, Qiu Ming;Gong, Jian Ping
• 通讯作者: Gong, Jian Ping

Local administration of synovium-derived fibroblasts cultured in TGF-betal-supple mented medium inhibits natural reduction of the structural properties of the tendon autograft after ACL reconstruction : A sheep model study

局部施用在添加 TGF-β 的培养基中培养的滑膜来源的成纤维细胞可抑制 ACL 重建后自体肌腱结构特性的自然降低：一项绵羊模型研究

• 发表时间: 2009
• 作者: Kondo E;Hayashi R;Katsura T;Azuma C;Tohyama H;Yasuda K
• 通讯作者: Yasuda K