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Study on Maternal Environmental Factors for Cleft Lip and/or Palate by Transplanting Fertilized Ova

受精卵移植引起唇腭裂的母体环境因素研究

基本信息

批准号：
12470452
负责人：
NATSUME Nagato
金额：
$ 3.07万
依托单位：
Aichi Gakuin University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12470452/
关键词：
cleft palate glucocorticoid(GCS)fertilized ovum transfer ddY mouse C57 BL mouse geneic engineering dexamethasone ddyマウス口唇裂ステロイドホルモン A Jマウス生殖細胞

项目摘要

Purpose : Existence of strain difference has been known in the study of glucocorticoids(GCS)-induced cleft palate mouse. Although the cause was pointed out to be fetsuse's genetic factors, a report also suggested maternal al factors. Past study used normal mating mouse. Fetal genetic factors and maternal factors were mixed, it was difficult to examine maternal factors among the different strains. In this study, we examined the maternal factors by fertilized ovum transfer that separated genetic factors and maternal factors.Materials and Methods : For donors of fertilized ovum, we used ddY mouse as the strain shows high occurrence rate of GCS-induced cleft palate. For receipient, we used ddY mice and C57BL/6J mice which have a lower occurrence rate of GCS-induced cleft palate. Occurrence rates were compared to normal mating ddY mouse group, C57BL/6J mouse group as experimental-1, GCS untreated groups with fertilized ovum transferred mouse group as experimental-2, and GCS treated groups w … More ith fertilized ovum transferred mice as experimental-3. Dex was treated in gestation day 11 and each group was observed in gestation day 18 and analyzed for occurrence rate of cleft palate. The data were analyzed by Fisher's exact test.Results : In experimental-1 group, the occurrence rate of cleft palate in ddY mouse was 96%, and in C57BL/6J mouse was20%. In experimental-2 group, the occurrence rate of cleft palate in both groups was 0%. In experimental-3 group, the occurrence rate of cleft palate in the group in which ddY mouse's fertilized ovum was transfered to the same strain showed 95%, and the group transfered to C57BL/6J mouse showed 29%. In experimental-1 group, the occurrence rates of GCS-indced cleft palate between ddY and C57BL/6J mouse by normal gestation were significantly different. The cleft palates of the experimental-3 group were assumed to be all induced by GCS because no occurrence of cleft palate was seen in the experimental-2 group. GCS-induced cleft palate was occurred by difference of metabolism ability at the mother's body and it was considered to be related strongly by maternal factors. Less

目的：在糖皮质激素（GCS）诱导的腭裂小鼠的研究中发现存在品系差异。虽然原因被指出是胎儿的遗传因素，但一份报告也提出了母亲的因素。过去的研究使用正常交配小鼠。胎儿遗传因素和母体遗传因素混合存在，不同菌株间的母体遗传因素难以检测。在这项研究中，我们通过分离遗传因素和母体因素的受精卵移植来检查母体因素。材料和方法：对于受精卵供体，我们使用ddY小鼠作为GCS诱导腭裂发生率高的品系。对于接受者，我们使用具有较低发生率的GCS诱导的腭裂的ddY小鼠和C57 BL/6 J小鼠。以正常交配ddY小鼠组、C57 BL/6 J小鼠组为实验1组、GCS未处理组加受精卵移植小鼠组为实验2组、GCS处理组加受精卵移植小鼠组为实验2组， ...更多信息第i个受精卵移植小鼠作为实验-3。在妊娠第11天给予地塞米松，在妊娠第18天观察各组并分析腭裂发生率。结果：实验1组ddY小鼠腭裂发生率为96%，C57 BL/6 J小鼠腭裂发生率为20%。实验-2组腭裂发生率为0%，对照组为0%。实验-3组中，ddY小鼠受精卵移植到同品系小鼠组腭裂发生率为95%，移植到C57 BL/6 J小鼠组腭裂发生率为29%。在实验1组中，正常妊娠的ddY和C57 BL/6 J小鼠的GCS诱导腭裂发生率有显著差异。实验-3组的腭裂被认为是由GCS诱导的，因为在实验-2组中没有观察到腭裂的发生。GCS所致腭裂的发生是由于母体代谢能力的差异，与母体因素有很强的相关性。少