Development of new cleft lip and/or palate mouse using genetic englnnering - Study on fertility of reproduction cell and hereditary system -

点击翻译按钮获取中文摘要

The purpose of this study is to make clear the cause of cleft lip and/or palate (CL/P) occurring in CL/P-prone A/J strain mouse. Incidence of CL/P in A/J mice obviously higher than in other strains and they inherit this characteristic for generations. Therefore, we studied about the causes of CL/P from genetic factor and maternal environmental factor respectively.We crossbred CL/P-resistant mice and CL/P-prone mice. Then observing embryos in the uteri, we studied about genetic and environmental factors. As a result, the number of placenta in CL/P-prone mice was low and that m CL/P-resistant mice was high regardless embryo's gene type. Also the rate of stillbirth and absorption was higher among embryos raised in the uteri of CL/P-prone mice. It was made clear that raising embryos in the uteri of CL/P-prone mice was more difficult than in CL/P-resistant mice. However, as there were no F1 embryos with CL/P raised in the uteri of CL/P-prone mice, it was suggested that maternal environmental factors were the cause of CL/P.Furthermore, by transplanting fertilized ova of CL/P-prone mice to uteri of CL/P-resistant mice, we observed the occurring of CL/P. As a result, CL/P occurred in embryos of CL/P-prone mice raised in the uteri of CL/P-resistant mice. This result suggested that genetic factor was the cause of CL/P rather than the environmental factor.In order to observe genetic factor experimentally, we put inherited gene of CL/P-prone mice into CL/P-resistant mice for generations to raise genetic ratio and observed the change of incidence. As a result, as genetic ratio of CL/P-prone mice raised, incidence raised. This result suggests that CL/P gene is transmitted to CL/P-resistant mice from CL/P-prone mice. At the same time, this mouse was admitted as a newly established CL/P model mouse, CCC to meet international standard.