Effects of peripheral muscle contraction and muscle sympathetic activity on the insulin sensitivity

外周肌肉收缩和肌肉交感神经活动对胰岛素敏感性的影响

• 批准号: 12480255
• 负责人: NAKAMURA Takao
• 金额: $ 4.8万
• 依托单位: Yamagata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12480255/
• 关键词: Insulin sensitivity; Euglycemic clamp; Blood glucose; Muscle contraction; Tetanic; Rhythmic; Rat; 高脂肪食; 糖尿病モデル; ED_<50>

Using normal and high-fat-fed (HFF) rats, by means of the euglycemic insulin clamp technique, we investigated insulin sensitivity during hind-limb muscle contraction as well as at rest, with and without sciatic nerve innervation. In addition, we measured hepatic glycogen contents, with a method using phenol and sulfuric acid, to distinguish endogenous and exogenous glucose used during the clamp, and separated endogenous rat insulin and exogenous human insulin with simultaneous measurements using human and rat plasma insulin assays. The results obtained suggest; (1) We accumulated basic data on insulin sensitivity to be used as the control in various studies in the future; (2) Commencement of insulin infusion prior to glucose infusion makes euglycemic clamp maneuver easier; (3) We established how to make HFF rats; (4) In HFF rats, ED_<50> of insulin is higher than in normal rats; (5) No significant change was observed in the insulin sensitivity during titanic muscle contraction; (6) Rhythmic contraction made the sensitivity higher; (7) Denervation considerably affects the insulin sensitivity at rest; (8) Glucose uptake mechanism after denervation may be different from that observed in normal rats; (9) The insulin sensitivity of HFF rats at rest was similar to that of normal rats after dennervation; (10) During the euglycemic clamp, endogenous hepatic glucose output was considerably larger than we expected, which may mean the effects of euglycemic clamp on glucose metabolism is large.

使用正常和高脂喂养（HFF）大鼠，通过正常葡萄糖胰岛素钳夹技术，我们研究了胰岛素敏感性在后肢肌肉收缩以及在休息时，有和没有坐骨神经支配。此外，我们测量了肝糖原含量，用苯酚和硫酸的方法，以区分内源性和外源性葡萄糖在钳，并分离内源性大鼠胰岛素和外源性人胰岛素，同时测量使用人和大鼠血浆胰岛素测定。结果表明，（1）建立了胰岛素敏感性的基础数据，为以后的研究提供了对照;（2）在葡萄糖输注前先输注胰岛素，使正常血糖钳夹操作更容易;（3）建立了HFF大鼠的制作方法;（4）HFF大鼠胰岛素的艾德_（ED）<50>高于正常大鼠;（5）肌收缩时胰岛素敏感性无明显变化，（6）节律性收缩使胰岛素敏感性增高，（7）去神经支配对静息时胰岛素敏感性有显著影响，（8）去神经支配对静息时胰岛素敏感性无明显影响。（8）去神经后葡萄糖摄取机制可能与正常大鼠不同：（9）静息状态下HFF大鼠胰岛素敏感性与去神经后正常大鼠相似;（10）正常葡萄糖钳夹过程中，内源性肝葡萄糖输出量明显大于预期，这可能意味着正常葡萄糖钳夹对葡萄糖代谢的影响很大。

项目成果

M.Kusunoki, K.Tsutsumi, T.Hara, H.Ogawa, T.Nakamura, T.Miyata, F.Sakakibara, Y.Fukuzawa, T.Suga, S.Kakumu, Y.Nakaya: "A lipoprotein activator, NO-1886 prevents impaired endohelium-dependent relaxation of aorta caused by exercise in aged rats."Exp. Geronto

M.Kusunoki、K.Tsutsumi、T.Hara、H.Okawa、T.Nakamura、T.Miyata、F.Sakakibara、Y.Fukuzawa、T.Suga、S.Kakumu、Y.Nakaya：“脂蛋白激活剂，NO

M.Kusunoki, et al.: "Correlation between lipid and glycogen contents in liver and insulin resistance in high fat-fed rats treated with the lipoprotein lipase activator NO-1886"Metabolism. (印刷中). (2002)

M.Kusunoki 等人：“用脂蛋白脂肪酶激活剂 NO-1886 治疗的高脂肪喂养大鼠肝脏中脂质和糖原含量与胰岛素抵抗之间的相关性”（出版中）。

T.Nakamura, et al.: "Microneurography for the measurement of muscle sympathetic nerve activity(MSNA) in anesthetized rats"Proc.JSBB(Yamagata). 79-82 (2002)

T.Nakamura 等人：“用于测量麻醉大鼠肌肉交感神经活动 (MSNA) 的显微神经造影”Proc.JSBB (Yamagata)。

Takao NAKAMURA: "The neural control of haemodynamics"Proc. 2nd Japan-Australia Cardiovasc. Bioeng Symp. (Sydney). 31 (2000)

Takao NAKAMURA：“血液动力学的神经控制”Proc。

M.Kusunoki, et al.: "Ethylicosapentate (omega-3 fatty acid) causes accumulation of lipids in skeletal muscle but suppresses insulin resistance in OLETF rats"Metabolism. 52. 30-34 (2003)

M.Kusunoki 等人：“乙基碳五酸（omega-3 脂肪酸）会导致骨骼肌中脂质积累，但会抑制 OLETF 大鼠的胰岛素抵抗”代谢。