Study on the glucose metabolism in skeletal muscle caused by peripheral sympathetic nerve stimulation

周围交感神经刺激引起的骨骼肌糖代谢研究

• 批准号: 17300139
• 负责人: NAKAMURA Takao
• 金额: $ 6.69万
• 依托单位: Yamagata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17300139/
• 关键词: Peripheral muscle; The sympathetic nervous system; Electrical stimulation; Insulin; glycogen; triglyceride; トリグリセリド

Types 1 and 2 diabetic model rats were made by administration of streptozotocin and by high fat feeding, respectively.Peripheral sympathetic fibers in the sciatic nerve were electrically stimulated using microneurographic technique to enhance the local sympathetic activity. A transient reduction of blood glucose, which implies an enhancement of glucose uptake in peripheral tissues, was observed without increase in plasma insulin in normal and diabetic model rats. In addition, such reduction was also observed in plasma triglyceride. Response of sympathetic nerve system to the glucose load was deteriorated in the diabetic model rats. After each experiment, muscle tissues were resected to measure glycogen and triglyceride contents. Glycogen contents in stimulation side were somewhat lower than contralateral side. Glucose transporters 1 and 4 and their messenger RNA levels did not show statistically significant change. Experimental protocol might be subject to change to clarify the effect.In addition to the original objectives, effects of the stimulation on lipid metabolism was suggested as well from the results shown above, Therefore, fatty acid distribution of fat tissues and so on was tried to measure. The results showed location dependency of fatty acids and different distribution between normal and diabetic model rats.These results may show the possibility of artificial control of peripheral glucose uptake via non-insulin mediated path including the sympathetic nervous system.

分别用链脲佐菌素和高脂饲料复制1型和2型糖尿病大鼠模型，用显微神经摄影技术电刺激坐骨神经周围交感神经纤维，增强局部交感神经活动。在正常和糖尿病模型大鼠中观察到血糖的短暂降低，这意味着外周组织中葡萄糖摄取的增强，而血浆胰岛素未增加。此外，在血浆甘油三酯中也观察到这种降低。糖尿病模型大鼠交感神经系统对糖负荷的反应性变差。每次实验后，切除肌肉组织以测量糖原和甘油三酯含量。刺激侧糖原含量略低于对侧。葡萄糖转运蛋白1和4及其信使RNA水平未显示统计学显著变化。实验方案可能会发生变化，以澄清效果。除了最初的目的，刺激对脂质代谢的影响也表明从上述结果，因此，脂肪组织的脂肪酸分布等，试图测量。结果显示脂肪酸的位置依赖性以及正常和糖尿病模型大鼠脂肪酸的不同分布，提示可能通过交感神经系统等非胰岛素介导的途径对外周葡萄糖摄取进行人工调控。

项目成果

末梢交感神経系機能の線維依存性に関する基礎的研究

周围交感神经系统功能纤维依赖性的基础研究

• 发表时间: 2007
• 期刊: 電気学会 電子・情報システム部門大会講演論文集 44
• 作者: 新澤剛;澤口明仁;楠正隆;中村孝夫
• 通讯作者: 中村孝夫

末梢交感神経系機能の線維依存性に関する基礎的研究(オーガナイズドセッション)

周围交感神经系统功能的纤维依赖性基础研究（组织会议）

• 发表时间: 2007
• 作者: 新澤剛;澤口明仁;楠正隆;中村孝夫
• 通讯作者: 中村孝夫

神経系を介した血糖制御の可能性(招請講演)

通过神经系统控制血糖的可能性（特邀讲座）

• 发表时间: 2006
• 作者: 中村孝夫;新澤剛;澤口明仁;楠正隆;鈴木隆司・佐藤実・佐藤大介・石井良一・楠正隆・中村孝夫;小野寿樹・石井良一・藤吉晶野・野村保友・中村孝夫;楠正隆・原田倫子・須賀敬・中村孝夫・小川斉・水谷浩也・普天間新生・内海恵子・古賀智明・清長聡子・三澤由美・鈴木綾・青木桂子・宮田哲郎;新澤剛・高橋智之・澤口明仁・楠正隆・中村孝夫;中村孝夫・楠正隆・新澤剛・高橋智之;中村孝夫
• 通讯作者: 中村孝夫

糖尿病患者におけるHOMA-Rと血中脂肪酸濃度の関連性

糖尿病患者HOMA-R与血脂肪酸浓度的关系

• 发表时间: 2006
• 期刊: 糖尿病 49・Su. lppl
• 作者: 楠正隆;原田倫子;須賀敬;中村孝夫;小川斉;水谷浩也;普天間新生;内海恵子;古賀智明;清長聡子;三渾由美;鈴木綾;青木桂子;宮田哲郎
• 通讯作者: 宮田哲郎

NO-1886(ibrolipim), a lipoprotein lipase activator, increases the expression of uncoupllng protein 3 in skeletal muscle and suppresses fat accumulation in high-fat diet-induced obesity in rats

NO-1886(ibrolipim) 是一种脂蛋白脂肪酶激活剂，可增加骨骼肌中解偶联蛋白 3 的表达并抑制高脂饮食诱导的肥胖大鼠中的脂肪积累

• 发表时间: 2005
• 期刊: Metabolism 54
• 作者: Kusunoki M;Tsutsumi K;Iwata K;Yin W;Nakamura T;Ogawa H;Nomura T;Mizutani K;Futenma A;Utsumi K;Miyata T
• 通讯作者: Miyata T