权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

PROGRAMMED CELL DEATH OF BURSAL LYMPHOCYTES INDUCED BY STEROID HORMONES

类固醇激素诱导法氏囊淋巴细胞程序性细胞死亡

基本信息

批准号：
05660325
负责人：
NAKAMURA Takao
金额：
$ 1.34万
依托单位：
GIFU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

项目摘要

The bursa of Fabricius is a lymphoid organ unique to avian species that is lacated dorsal to the cloaca. The chiken bursa decreases in sizu when the bird is subjected to stresses such as handling, vaccination and high-density rearing. Glucocorticoid secreted from the adrenal cortex during exposure to stress is known to cause apoptosis of bursal lymsphocytes, with resultant involution of the bursa. Furthermore, the bursa undergoes spontaneous regression when sexual maturation occurs. This study was undertaken in an attempt to clarify the role of steroid hormones in apoptosis of bursal lymphocytes by administering various steroids.Four-week-old male chickens were given a single injection of 1mg/kg body weight of dexamethasone, 2mg/kg of estradiol banzoate, 4mg/kg of testosterone or 2mg/kg of testosterone propionate and they were sacrificed 1,2 or 4 hr later. DNA was prepared from bursal tissue and subjected to electrophoresis in a 1.8% agarose gel.A "ladder pattern" on the electrophoretogram, which is a biochemical hallmark of apoptosis, was obtained from the following treated chickens : those treated with dexamethasone, estradiol benzoate, testosterone and testosterone propionate. It seems possible, from these results, that apoptosis might be induced by sex steroids during spontaneous regression of the chicken bursa. In another series of experiments, ubiquitin was detected immunohistochemically in apoptotic bursal lymphocytes 2 and 48 hr after an injection of dexamethasone, a result that suggests the possible involvement of ubiquitin in bursal apoptosis.

法氏囊是鸟类特有的淋巴器官，位于泄殖腔的背侧。当鸡受到处理、接种疫苗和高密度饲养等压力时，鸡的囊变小。已知应激时肾上腺皮质分泌的糖皮质激素会导致法氏囊淋巴细胞凋亡，导致法氏囊内陷。此外，当性成熟发生时，囊会自发退化。本研究旨在阐明类固醇激素在法氏囊淋巴细胞凋亡中的作用。4周龄雄鸡单次注射地塞米松1mg/kg体重、苯甲酸雌二醇2mg/kg体重、睾酮4mg/kg体重或丙酸睾酮2mg/kg体重，1、2、4小时后处死。从法氏囊组织中制备DNA，并在1.8%琼脂糖凝胶中进行电泳。电泳图上的“阶梯图”是细胞凋亡的生化标志，从以下处理的鸡中获得：地塞米松、苯甲酸雌二醇、睾酮和丙酸睾酮。从这些结果来看，似乎有可能在鸡法氏囊自发退化过程中，性类固醇可能诱导细胞凋亡。在另一系列实验中，在注射地塞米松后2和48小时，免疫组织化学检测到泛素在法氏囊凋亡淋巴细胞中，结果提示泛素可能参与法氏囊凋亡。