Effects of polyunsaturated fatty acids in medium on cultured cardiomyocyte function

对胎儿、新生儿和培养心肌细胞（CMs）的脂肪酸（FA）组成进行了评估和比较。结果表明，培养的CMs中多不饱和脂肪酸（PUFAs）的含量明显低于新生儿。因此，我们在培养基中加入了一些与白蛋白结合的PUFAs，发现FAs成功地掺入到CMs中，FA的延伸可能发生了，并且发泡性能提高了三倍。下一阶段应优化fa的添加量，以获得最佳力学性能。

Fatty acid (FA) compositions in fetal, neonatal, and cultured cardiomyocytes (CMs) were evaluated and compared to each other. The results suggest that cultured CMs have considerably lower amount of polyunsaturated FAs (PUFAs) than neonatal ones do. Thus, we added some PUFAs, which were combined with albumin, into medium, and found that FAs were successfully incorporated in CMs, that FA elongation may have taken place, and that the beating performance was tripled. In the next stage, FAs to add should be optimized to gain the best mechanical performance.