Liquid ventilation as a bridging to lung transplantation

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There has been no applicable ventilation devises to support patients with chronic with respiratory failure. In the present research project, we sought to evaluate whether liquid ventilation using across gut and/or peritoneum would be function to increase oxygenation. We utilized FC-77 in not only cell experiments, but also anesthetized rat models. In cell experiments, we mixed oxygenated FC-77 with human peripheral venous blood in a test tube. We found a marked increase in Pao2 and a decrease in PaCO2 in the first minute following mixture. We also found that FC-77 was able to deliver O2 and absorb CO2 across the monolayers of human pulmonary arterial endothelial cells and human alveolar epithelial cells (A549) in the transwell insert systems. In rat experiments, rats were anesthetized and ventilated with low tidal volume (VT=5ml/kg) at low ventilation rate (f=20), and then oxygenated FC-77 (20ml/kg) was introduced into colon or peritoneal cavity. We found a progressive increase in PaO2 in both colon and or peritoneum experiments over 4 hours following administration of FC compound. However, peritoneal administration seemed to be somewhat superior not only in oxygenation, but also in the ability of removing C02. We believe that FC-77 would be applicable in the rescue of patients with chronic with respiratory failure. Our data obtained in this project indicate that liquid ventilation across the peritoneum would potentially become possible in near future.