Elucidation of Molecular Mechanism of Cell Death induced by Radiation, Hyperthermia and Ultrasound for Cancer Therapy

阐明放射线、热疗和超声治疗癌症时诱导细胞死亡的分子机制

• 批准号: 22390229
• 负责人: KONDO Takashi
• 金额: $ 8.49万
• 依托单位: University of Toyama
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22390229/
• 关键词: 放射線腫瘍学; 超音波治療学; 温熱治療学; ハイパーサーミア; アポトーシス; 放射線; 超音波; TAK1; Chk1; 遺伝子発現; がん; 細胞死; 治療

Molecular mechanism of cell death induced by ionizing radiation, heat or ultrasound was examined. Stable knockdown of TAK1 caused increase in subG1 fraction and apoptosis induced by X-rays in HeLa cells. Inhibition of DNA-PK increased heat-induced apoptosis in HeLa cells and the knockdown of an identified gene, NR1D1, also enhanced apoptosis. For the cell death induced by ultrasound, cell death signal is modulated by the balance between p53 and Akt via ATM and DNA-PK and the pathway of DNA-PK/Akt is predominant in sonicated cells.

研究了电离辐射、热或超声诱导细胞死亡的分子机制。稳定敲除TAK 1可引起HeLa细胞亚G1期细胞比例的增加和X射线诱导的细胞凋亡。DNA-PK的抑制增加了HeLa细胞中热诱导的细胞凋亡，并且已鉴定的基因NR 1D 1的敲除也增强了细胞凋亡。在超声波诱导的细胞死亡中，细胞死亡信号主要通过ATM和DNA-PK调节p53和Akt之间的平衡，其中DNA-PK/Akt途径占主导地位。

项目成果

Ionizing Radiation Enhances Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand (TRAIL)-Induced Apoptosis through Up-Regulations of Death Receptor 4 (DR4) and Death Receptor 5 (DR5) in Human Osteosarcoma Cells

• DOI: 10.1002/jor.21056
• 发表时间: 2010-06-01
• 期刊: JOURNAL OF ORTHOPAEDIC RESEARCH
• 影响因子: 2.8
• 作者: Hori, Takeshi;Kondo, Takashi;Kimura, Tomoatsu
• 通讯作者: Kimura, Tomoatsu

Mitochondria-Targeted Superoxide Dismutase (SOD2) Regulates Radiation Resistance and Radiation Stress Response in HeLa Cells

• DOI: 10.1269/jrr.11034
• 发表时间: 2012-01-01
• 期刊: JOURNAL OF RADIATION RESEARCH
• 影响因子: 2
• 作者: Hosoki, Ayaka;Yonekura, Shin-Ichiro;Zhang-Akiyama, Qiu-Mei
• 通讯作者: Zhang-Akiyama, Qiu-Mei

TGF-beta-activated kinase 1 promotes cell cycle arrest and cell survival of X-ray-irradiated HeLa cells dependent on p21 induction but independent of NF-kB, p38 MAPK and ERK phosphorylations

TGF-β 激活激酶 1 促进 X 射线照射的 HeLa 细胞的细胞周期停滞和细胞存活，依赖于 p21 诱导，但不依赖于 NF-kB、p38 MAPK 和 ERK 磷酸化

• DOI: 10.1667/rr2792.1
• 发表时间: 2012
• 期刊: Radiat Res
• 影响因子: 3.4
• 作者: Furusawa Y;Wei ZL;Sakurai H;Tabuchi Y;Li P;Zhao Q-L;Zhao;Nomura T;Saiki I;and Kondo T
• 通讯作者: and Kondo T

Hyperthermia enhances the effect of β-lapachone to cause γH2AX formation and cell death in human osteosarcoma cells

热疗增强β-拉帕酮引起人骨肉瘤细胞γH2AX形成和细胞死亡的作用

• 发表时间: 2011
• 期刊: Int J Hyperthermia
• 作者: Hori T.;et al.
• 通讯作者: et al.

Ultrasound-induced DNA damage and signal transductions indicated by gammaH2AX

gammaH2AX 表明超声诱导的 DNA 损伤和信号转导

• DOI: 10.1063/1.3607926
• 发表时间: 2011
• 作者: Yukihiro Furusawa;Y. Fujiwara;Qing‐Li Zhao;M. Hassan;R. Ogawa;Y. Tabuchi;I. Takasaki;A. Takahashi;T. Ohnishi;T. Kondo
• 通讯作者: T. Kondo