Allograft Rejection in FcR γ knockout mice

为了阐明Fc受体在同种异体移植排斥反应过程中的作用，在FcR γ敲除小鼠中进行同种异体移植。采用MHC不相容小鼠（BALB/c→ C57 BL/6）进行皮肤移植。C57 BL/6小鼠是FcR γ缺陷的小鼠或野生型对照。FcR γ基因敲除小鼠和对照小鼠的皮肤移植物均在移植后7或8天内发生排斥反应，两组之间的移植物存活时间没有差异。接下来，为了研究在细胞表面上表达Fc受体的巨噬细胞在同种异体移植物排斥中的作用，在大鼠的MHC不相容组合中进行异种气管移植。在第0、7、14天给SD大鼠注射GdCl 3，其具有消耗巨噬细胞的能力，并在第21天收获气管同种异体移植物。阻塞性气道疾病的严重程度，这是已知的发展与同种异体移植物排斥反应，显着降低GdCl 3治疗的动物相比，对照组。这表明巨噬细胞在大鼠异位气管移植模型中同种异体排斥反应和阻塞性气道疾病的发展过程中起作用。

To clarify the role of Fc receptors in the process of allograft rejection, allotransplantations were performed in FcR γ knockout mice. Skin transplantation was performed between a MHC incompatible combination of mouse (BALB/c→C57BL/6). Recipient C57BL/6 mice were those deficient in FcR γ or wild-type controls. Skin allografts in FcR γ knockout mice and the controls were both rejected within day 7 or 8 post-transplant and there was no difference in the duration of graft survival between the groups. Next, to investigate the role of macrophages that express Fc receptors on cell surface in allograft rejection, heteropic tracheal transplantation was performed in a MHC incompatible combination of rat. Recipient rats were injected with GdCl3, which has an ability to deplete macrophages, on day 0, 7, 14 and tracheal allografts were harvested on day 21. The severity of obstructive airway disease, which is known to develop in association with allograft rejection, was significantly reduced in GdCl3-treated animals compared to controls. This indicates that macrophages play a role in the process of allograft rejection and the development of obstructive airway disease in a model of heterotopic tracheal transplantation in rats.