Experimental study on the assessment of lung preservation from the stand point of the alveolar type II epithelial cell function

本文用充液大鼠肺模型，以肺泡液体清除率为指标，对肺保存条件进行了评价，并获得了以下结果。(1)EP 4液保存24 h的效果明显优于Euro-Cllins液保存的上级效果。(2)尽管效果良好，但即使用EP 4液保存肺24小时，特布他林对Na-K-ATP酶功能的增强作用也被证明是受损的。(3)地塞米松在给药后72小时证明可增强Na-K-ATP酶功能，这证明了类固醇激素在肺保护中的有益作用。(4)用培养的肺泡上皮细胞进行冷保存，发现EP 4液中IL 8的产生低于Euro-Collins液中IL 8的产生，而在培养液中加入地塞米松后，IL 8的产生得到改善，这一结果表明EP 4液在肺移植模型中的优越性。此外，肺移植术后再灌注损伤的类固醇治疗被证明是适当的，从培养的肺泡上皮细胞的IL 8评估的结果。基于这些基本结果，可以预期肺保护的进一步改进。

Conditions of pulmonary preservation have been assessed by the alveolar fluid clearance with the fluid-filled rat lung model and following results have been obtained sofar.(1) 24-hour preservation with EP4 solution showed significantly superior result compared with that with Euro-Cllins solution.(2) In spite of good result, the functional enhancement of Na-K-ATPase by Terbutaline was demonstrated to be damaged even if the lung was preserved for 24 hours with EP4 solution.(3) Dexamethazone proved to enhance the Na-K-ATPase function 72 hours after administration, which demonstrated the beneficial effect of steroid hormone in pulmonary preservation.(4) Using cultured alveolar epithelial cells, IL8 production after the exposure of the cold preservation found to be lower in EP4 solution than in Euro-Collins solution, which was improved by the supplement of Dexamethazone in the cultured solution.These results indicate the superiority of EP4 solution in pulmonary preservation which has been suggested by canine or primate lung transplantation models. Also, steroid treatment for the reperfusion injury after lung transplantation proved to be appropriate from the result of IL8 assessment by cultured alveolar epithelial cell. Based on these basic results, further improvement of pulmonary preservation can be expected.