权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Exhausitive search for the identification of lung cancer specific gene splicing by microarray technique

微阵列技术穷举鉴定肺癌特异性基因剪接

基本信息

批准号：
15390407
负责人：
KONDO Takashi
金额：
$ 9.54万
依托单位：
Tohoku University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15390407/
关键词：
small peripheral lung adenoca.GGO CT M L ratio TIMP-2 Ki-67 Methylation microarray スプライシング肺癌 CT K-ras 細胞株スプライス

项目摘要

Peripheral small lung adenocarcinomas have been detected more frequently in these days according as CT screening has become popular. The computed tomographic M/L ratio (area of the tumor in the mediastinal computed tomographic image/area of the tumor in the lung computed tomographic image) of small peripheral lung adenocarcinoma is correlated with patient prognosis. The numbers of patients with high labeling indexes of Ki-67 and high expression of vascular endothelial growth factor, CD34, matrix metalloproteinase2, and matrix metalloproteinase9 in the solid-type group (computed tomographic M/L ratio >50%) are significantly higher than those in the faint density-group (computed tomographic M/L ratio <50%). The number of patients with high levels of CD44v6 or tissue inhibitor of matrix metalloproteinase2 staining in the faint density-type group is significantly higher than that in the solid-type group. Independent variables capable of predicting computed tomographic M/L ratio included CD34, matric metalloproteinase 2, matrix metalloproteinase 9, and tissue inhibitor of matrix metalloproteinase 2.Aberrant methylation of DNA is an important event in carcinogenesis. A microarrray analysis of DNA methylation in the promoter regions of genes using a newly developed promoter-associated methylated DNA amplification DNA chip. About 10.9% of the cancer-related genes are hypermethylated in lung cancer cell lines. Notably, HIC1, IRF7, ASC, RIPK3, RASSF1A, FABP3, PRKCDBP, and PAX3 genes are hypermethylated in most lung cancer cell lines examined. The expression profiles of these genes correlated to the methylation profiles of the genes, indicating that the microarray analysis of DNA methylation in the promoter region of the genes is convenient for epigenetic study.

近年来，随着CT筛查的普及，周围型小肺腺癌的检出率越来越高。周围型小肺腺癌的计算机断层扫描M/L比（纵隔计算机断层扫描图像中的肿瘤面积/肺部计算机断层扫描图像中的肿瘤面积）与患者的预后相关。实体型组（CT M/L比值>50%）Ki-67高标记指数和血管内皮生长因子、CD34、基质金属蛋白酶2、基质金属蛋白酶9高表达的患者数量显着高于微密度组（CT M/L比值<50%）。微密度型组中CD44v6或基质金属蛋白酶组织抑制剂2染色水平较高的患者数量明显高于实心型组。能够预测计算机断层扫描M/L比率的自变量包括CD34、基质金属蛋白酶2、基质金属蛋白酶9和基质金属蛋白酶2的组织抑制剂。DNA的异常甲基化是癌发生中的重要事件。使用新开发的启动子相关甲基化 DNA 扩增 DNA 芯片对基因启动子区域的 DNA 甲基化进行微阵列分析。肺癌细胞系中约 10.9% 的癌症相关基因高甲基化。值得注意的是，HIC1、IRF7、ASC、RIPK3、RASSF1A、FABP3、PRKCDBP 和 PAX3 基因在大多数检查的肺癌细胞系中均高度甲基化。这些基因的表达谱与基因的甲基化谱相关，表明基因启动子区DNA甲基化的微阵列分析便于表观遗传学研究。