An attempt to promote formation of neural network on dental implants

1. 神经细胞在钛板上存活的研究在培养液中加入神经生长因子，可延长神经细胞PC12细胞在钛板上的胞质过程。然而，这些细胞很容易从钛板表面脱落。这些发现表明神经纤维不能附着在钛上。采用电镜和组织化学技术研究了大鼠上颌组织对钛植入的反应。实验数据表明，大鼠上颌骨钛种植体周围骨化的方式不同，取决于受体骨的性质和种植体与截骨边缘间隙的大小。植体上皮内神经纤维的再生。采用大鼠上颌骨和免疫组化技术建立大鼠种植体周围上皮神经纤维对钛植入的反应。实验数据表明，种植体周围上皮的神经支配与正常的连接上皮相同，并且种植体周围上皮的上皮内神经纤维可能具有文献所述的多种功能。种植体表面状况与骨形成过程的关系。采用光镜、透射电镜和组织化学方法研究了两种不同表面条件下大鼠上颌钛植入模型的组织反应。我们使用了两种不同表面质量的种植体：A1203喷砂钛种植体（sa组）和羟基磷灰石涂层种植体（ha组）。我们的数据表明，钛种植体周围的骨化以不同的模式发展，可能取决于表面特性和质量。

1. Finding on the survival of neuronal cells on titanium plateBy adding nerve growth factor in culture medium, PC12 cells, neuronal cells, could extend their cytoplasmic processes on titanium plate. However, these cells easily exfoliated from the surface of titanium plate. These findings suggest that nerve fibers cannot adhere to titanium.2. Tissue response to titanium implantation in rat maxillaTissue response to titanium implantation was investigated in an animal model using maxilla by use of eletron microscopy and histochemical technique. The experimental data indicated that ossification proceeded at different modes around the titanium implant in rat maxilla, depending on the nature of the recipient bones and the dimension of the gap between the implant and osteotomy margin.3. Regeneration of nerve fibers in the per-implant epitelium.The response of nerve fibers in the peri-implant epithelium to titanium implantation was investigated with an experimental model using rat maxilla and immunohistochemical techniques. The experimental data indicated that the peri-implant epithelium showed the same innervation to that in normal junctional epithelium, and that the intraepitelial nerve fibers in the peri-implant epithelium might have diverse functions which have been suggested in the literature.4. Relationship between the surface conditon of implant and bone formation process.Tissue responses to titanium implantation with two different surface conditions in our established implantation model in rat maxillae were investigated by light and transmission electron microscopy and by histochemistry for tartrate resistant acid phosphatase activity. We here used two types of implants with different surface qualities : titanium implants sandblasted with A1203 (SA-group), and implants coated with hydroxyapatite (HA-group). Our data indicated that ossification around the titanium implants progressed in differ ent patterns, probably dependent on surface properties and quality.