THE INVOLVEMENT OF BRAIN DERIBED NEUROTROPHIC FACTOR (BDNF) IN DEVELOPMENT<REGENERATION AND MAINTENACE OF THE PERIODONTAL RUFFINI ENDINGS-A STUDY USING BDNF KNOCKOUT MICE-

脑源性神经营养因子 (BDNF) 参与牙周鲁菲尼末梢的发育和再生-使用 BDNF 敲除小鼠的研究-

• 批准号: 14370580
• 负责人: MAEDA Takeyasu
• 金额: $ 9.02万
• 依托单位: NIIGATA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14370580/
• 关键词: Ruffini ending BDNF; knockout mice; development; neurotrophic factor; regeneration; image analysis; innervation; 神経支配; 歯根膜; 神経終末; PGP 9.5

The present study examined the terminal formation and regeneration/development of the periodontal Ruffini endings in the BDNF knockout (homozygous and heterozygous) and wild type mice by immunocytochemical method. The main results obtained in this study are as follows.1.The periodontal ligament of wild type types ; Ruffini nerve endings with irregular-(type I) and smooth outlines (type II). The periodontal ligament of heterozygous mice had less development and malformation of type I, which fewer arborizations and less expansion of the axon terminals. Image analysis demonstrated a 18% reduction of neural density in wild type than in heterozygous mice.2.Observation from postnatal 1 to 3 weeks showed significant difference in neural density between homozygous mice and others.3.An experimental study on the resection of inferior alveolar nerve demonstrated that a depletion of BDNF induced a delay in recovery of the regeneration of the periodontal Ruffini endings, suggesting that BDNF plays crucial roles in the regeneration process of these endings.In conclusion, our study supported by this grant was clearly able to demonstrate the involvement of BDNF in the regeneration and development, in particular maturation stage, of the periodontal Ruffini endings.

本研究采用免疫细胞化学方法研究了BDNF敲除（纯合和杂合）和野生型小鼠牙周Ruffini末梢的形成和再生/发育。本研究得到的主要结果如下：1。野生型牙周韧带；鲁菲尼神经末梢不规则（I型），轮廓光滑（II型）。杂合小鼠的牙周膜发育和畸形较少，即轴突末端的屈曲和扩张较少。图像分析显示，野生型小鼠的神经密度比杂合子小鼠减少18%。产后1 ~ 3周观察，纯合子小鼠的神经密度与其他小鼠有显著差异。一项关于下牙槽神经切除的实验研究表明，BDNF的缺失导致牙周Ruffini末梢再生的恢复延迟，这表明BDNF在这些末梢的再生过程中起着至关重要的作用。综上所述，这项资助支持的研究能够清楚地证明BDNF参与牙周Ruffini末梢的再生和发育，特别是成熟阶段。

项目成果

Hoshino, N.: "Involvement of brain-derived neurotrophic factor (BDNF) in the development of periodontal Ruffini endings."Anat.Rec.. 274A. 807-816 (2003)

Hoshino, N.：“脑源性神经营养因子 (BDNF) 参与牙周鲁菲尼末梢的发育。”Anat.Rec.. 274A。

B.A.Alkhamrahほか: "The Periodontal Ruffini Endings in the Brain Derived Neurotrophic Factor (BDNF) Deficient Mice"Archives of Histology and Cytology. 66・1. 73-81 (2003)

B.A. Alkhamrah 等：“脑源性神经营养因子 (BDNF) 缺陷小鼠的牙周 Ruffini 结局”，组织学和细胞学档案 73-81。

Harada, F.: "The involvement of brain-derived neurotrophic factor (BDNF) in the regeneration of the periodontal Ruffini endings following transaction of the inferior alveolar nerve."Arch.Histol.Cytol.. 66・2. 183-194 (2003)

Harada, F.：“脑源性神经营养因子 (BDNF) 在下牙槽神经切除后牙周 Ruffini 末梢的再生中的作用。”Arch.Histol.Cytol.. 66・2 (2003) ）

Byers, M.R.: "Dental neuroplasticity, neuro-pulpal interactions and nerve regeneration."Microsc.Res.Tech.. 60・5. 503-515 (2003)

Byers, M.R.：“牙科神经可塑性、神经牙髓相互作用和神经再生”。Microsc.Res.Tech.. 60・5 (2003)。

Iijima, K.et al.: "Temporal expression of immunoreactivity for heat shock protein 25 (Hsp25) in the rat periodontal ligament following transection of the inferior alveolar nerve."Brain Res.. 979. 146-152 (2003)

Iijima, K.等人：“下牙槽神经横断后大鼠牙周膜中热休克蛋白 25 (Hsp25) 免疫反应性的时间表达。”Brain Res.. 979. 146-152 (2003)