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Asurvey of new factors which involve in development and regeneration process of the periodontal Ruffini endings

牙周鲁菲尼末梢发育和再生过程新因素的探讨

基本信息

批准号：
18390488
负责人：
MAEDA Takeyasu
金额：
$ 11.01万
依托单位：
Niigata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18390488/
关键词：
GDNF GDNF receptor periodontal ligament Ruffini ending galaectin ルフィ二神経終末ガレクチン

项目摘要

Our previous studies haw revealed an immuno-expression of trk B, a high affinity neurotrophin receptor in the periodontal ligament. Furthermore, we have shown the involvement of BDNF and NT-4/5, ligands for trkB, in the development/regeneration of the periodontal Ruffini endings. However, no information available for the molecules involved in their maturation process to date. Thus, the present study was undertaken to investigate expression of GDNF (glial-cell line derived neutrotrophic factor) and galectin in the periodontal Ruffini endings of the rat incisors. The main results obtained from this study are as follows.1. APCR analysis detected intense signals far GDNF, GFRαl and Ret mRNAs in the trigeminal ganglion. Immunocytochemical analysis demonstrated positive reaction for GFRαl and Ret in about 30% trigeminral neurons, almost which were categorized in medium-sized neurons that mediate mechanotransduction by an image analysis. GDNF-immunoreaction was localized infix satellite cells … More , never in the neurons.2. The mature periodontal Ruffini endings exhibited GDNF, GFRαl and Ret-immunoreactions in the terminal Schwann cells. However, their axon terminals were devoid of GDNF-immunoreaclion. Furthermore, no immunoreaction existed in the periodontal Ruffini endings.3. The periodontal Ruffini endings lacked all kinds of immunoreaction at postnatal 3 days. At postnatal week 1, the terminal Schwarm cells became positive for GFRαl. Apart of the axon terminals showed Ret-immunoreaction at week 2, and a few GDNF-reactive terminal Schwann cells occurred near the thickening axon terminals. Thereafter, the GDNF-positive terminal Schwann cells increase in number.4. An experiment to resect inferior alveolar nerve showed first appearance of the GDNF-positivity in the terminal Schwann cells, spindle-in shape, at postoperative 2 weeks. Maine terminal Schwann cells which had round profile were detectable as GDNF-immunopositive after postoperative 3 weeks. These stage-specific expression patterns might be related to the maturation and maintenance of the periodontal Ruffini endings. Less

我们之前的研究揭示了牙周膜中高亲和力神经营养蛋白受体 trk B 的免疫表达。此外，我们还表明 BDNF 和 NT-4/5（trkB 的配体）参与牙周 Ruffini 末端的发育/再生。然而，迄今为止，还没有关于参与其成熟过程的分子的信息。因此，本研究旨在研究大鼠门牙牙周鲁菲尼末梢中 GDNF（胶质细胞系衍生的中性营养因子）和半乳糖凝集素的表达。本研究取得的主要结果如下： 1． APCR 分析检测到三叉神经节中 GDNF、GFRα1 和 Ret mRNA 的强烈信号。免疫细胞化学分析表明，约30%的三叉神经元中GFRα1和Ret呈阳性反应，通过图像分析，这些神经元几乎被归类为介导机械转导的中等大小的神经元。 GDNF-免疫反应是定位于卫星细胞中的，而不是在神经元中。2。成熟的牙周Ruffini末端在终末雪旺细胞中表现出GDNF、GFRα1和Ret免疫反应。然而，它们的轴突末端缺乏 GDNF 免疫反应。此外，牙周Ruffini末梢不存在免疫反应。3．出生后3天牙周Ruffini末梢缺乏各种免疫反应。出生后第 1 周，终末 Schwarm 细胞对 GFRα1 呈阳性。第2周时，部分轴突末端出现Ret免疫反应，增厚的轴突末端附近出现一些GDNF反应性末端雪旺细胞。此后，GDNF阳性末端雪旺细胞数量增加。4．一项切除下牙槽神经的实验显示，术后 2 周，终末雪旺细胞中首次出现 GDNF 阳性，呈纺锤形。术后 3 周后，可检测到呈圆形轮廓的缅因州终末雪旺细胞，呈 GDNF 免疫阳性。这些阶段特异性表达模式可能与牙周鲁菲尼末端的成熟和维持有关。较少的