权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Immunohistochemical and Electron Microscopic Study on the Somatosensory Receptive System in Orofacial Region

口面部体感感受系统的免疫组织化学和电镜研究

基本信息

批准号：
63440069
负责人：
MAEDA Takeyasu
金额：
$ 18.37万
依托单位：
Niigata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (A)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1990
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63440069/
关键词：
Innervation Dental pulp Periodontal ligament Immunohistochemistry Ruffini ending Neurofilament protein Calcitonin geneーrelated peptide (CGRP)Nerve growth factor receptor ルフィニ小体立体構築 CGRP 顎関節矯正力ラット歯根膜 Ruffini神経終末 terminal S

项目摘要

The findings obtained from this study are summarized as follows.1. A major population of pulpal nerves in human teeth showed an intense nerve growth factor receptor (NGFR)-immunoreactivity. Immunoelectron microscopy demonstrated that the NGFR-immunoreactivity was localized on the axoplasmic membrane in both unmyelinated and naked axons, but not in myelinated fibers. A positive immunoreaction was also found in Schwann cells in unmyelinated fibers. Fibroblasts, cellular elements other than axons and some of Schwann cells were immunonegative.2. Despite variation in development among species, Ruffini endings are accepted as essential mechanoreceptors, sensitive to tension of collagen fibers, in the periodontal ligament. Ruffini endings in the periodontal ligament were associated with terminal Schwann cells whose cytoplasm contained wellーdeveloped rough endoplasmic reticulum and Golgi apparatus. In addition to ordinary functions of supporting and nourishing of axon terminals, these cells we … More re able to synthesize cholinesterase and proteoglycans.3. The response of pulpal nerves to dentin injury was investigated in rat molars by immunostaining for neurofilament protein. The distribution and shape of pulpal nerves reacted dynamically in response to cavity preparations, indicating possible involvement of the pulpal nerves in the tissue repair process following cavity preparations.4. The response of the periodontal nerve fibers to orthodontic tooth movement was investigated in rat molars by use of immunohistochemistry for calcitonin geneーrelated peptide (CGRP). The dynamic changes of CGRP-immunoreactive nerves in the periodontal ligament was recognized during the tooth movement. It is suggested that the periodontal nerves are involved in inducing pain sensation in patients.5. Although the presence of a variety of mechanoreceptors in the temporomandibular joint has been suggested by electrophysiological studies, no specialized nerve terminals except free nerve endings were observed in this immunohistochemical study. Less

本研究的主要结果如下：1.在人类牙齿牙髓神经的主要人口表现出强烈的神经生长因子受体（NGFR）的免疫反应。免疫电镜显示NGFR免疫反应位于无髓和裸轴突的轴质膜上，但不在有髓纤维中。无髓纤维中的雪旺细胞也呈阳性反应。成纤维细胞、轴突以外的细胞成分和部分雪旺细胞呈免疫阴性.尽管物种之间的发展变化，Ruffini结束被认为是必不可少的机械感受器，敏感的胶原纤维的张力，在牙周膜。牙周膜中的鲁菲尼终末与终末施万细胞相关，后者的细胞质含有发育良好的粗面内质网和高尔基体。这些细胞除了支持和滋养轴突终末的一般功能外， ...更多信息能够合成胆碱酯酶和蛋白多糖.采用免疫组化方法观察牙髓神经对牙本质损伤的反应。牙髓神经的分布和形态对洞型预备的反应是动态的，提示牙髓神经可能参与洞型预备后的组织修复过程.应用降钙素基因相关肽（CGRP）免疫组织化学方法，观察大鼠磨牙牙周神经纤维对正畸牙移动的反应。牙周膜内CGRP免疫反应阳性神经在牙齿移动过程中呈动态变化。提示牙周神经参与了疼痛感觉的产生.虽然在颞下颌关节的各种mechanoreceptors的存在已被建议的电生理学研究，没有专门的神经末梢，除了游离神经末梢，观察在本免疫组化研究。少