Conservation and Analyzes of Genetic Information of Asian Ethnic Groups

亚洲族群遗传信息的保存与分析

• 批准号: 12575015
• 负责人: ISHIDA Takafumi
• 金额: $ 10.11万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12575015/
• 关键词: Asia; Ethnic Group; Genetic Information; Cell bank; DNA bank; DNA; 少数民族; 細胞株; 霊長類; 多様性

The aim of this study is to conserve genetic information of Asian ethnic groups especially small sized populations in the form of a cell bank and DNA bank. For this, we have conducted field studies in Indonesia, Malaysia and Thailand.1. Field study: for the population genetic study, specimens were collected from Sumbanese, Timorese, Moken and Mani. A total of 400 specimens were collected and 250 cell lines were established.2. Study on Genome diversity: Polymorphisms of the following genes, SDF1, MC1R, AIMland p53, were studied among these populations with other Asian and Oceanian populations.3. Malaria related genes: Prevalence of 27-bp deletion of the 3 protein gene arid G6PD deficiency was studied in Sumbanese in the presence of the parasitemia data.4. Epstein-Barr virus associated peripheral T-cell proliferative diseaseis: A rapid and sensitive plasma DNA test for this disease entity was established.

本研究的目的是以细胞库和DNA库的形式保存亚洲少数民族特别是小群体的遗传信息。为此，我们在印度尼西亚、马来西亚和泰国进行了实地研究。野外研究：在种群遗传研究中，收集了来自桑班纳、帝汶、莫肯和马尼的标本。共收集标本400份，建立细胞系250株。基因组多样性研究：研究了这些人群与其他亚洲和大洋洲人群中SDF1、MC1R、aimand p53基因的多态性。疟疾相关基因：在存在寄生虫病数据的情况下，研究了3蛋白基因27 bp缺失和G6PD缺乏症在Sumbanese人中的流行情况。eb病毒相关外周t细胞增生性疾病：建立了一种快速灵敏的血浆DNA检测方法。

项目成果

Kimura, R. et al.: "The SDF1-G801A polymorphism is not associated with SDF1 gene expression in Epstein-Barr virus-transformed lymphoblastoid cells"Genes and Immunity. (in press). (2003)

Kimura, R. 等人：“SDF1-G801A 多态性与 Epstein-Barr 病毒转化的淋巴母细胞中的 SDF1 基因表达无关”《基因与免疫》。

Shimizu,Y., et al.: "Sero-and Molecular Typing of Duffy Blood Group in Southeast Asians and Oceanians."Human Biology. 72(3). 511-518 (2000)

Shimizu,Y., et al.：“东南亚和大洋洲人达菲血型的血清和分子分型。”人类生物学。

Mori, A., Takao, S.et al.: "High tumor necrosis factor-a levels in the patients with Epstein-Barr virus-associated peripheral T-cell proliferative disease/lymphoma"Leukemia Research. 27. 493-498 (2003)

Mori, A.、Takao, S.等人：“Epstein-Barr 病毒相关外周 T 细胞增殖性疾病/淋巴瘤患者的肿瘤坏死因子 -a 水平较高”白血病研究。

Kimura, M., Soemantri, Ag., Ishida, T.: "Malaria species and Southeast Asian ovalocytosis defined by a 27-bp deletion in the erythrocyte band 3 gene"Southeast Asian Journal of Tropical Medicine and Public Health. 33. 4-6 (2002)

Kimura, M.、Soemantri, Ag.、Ishida, T.：“疟疾种类和东南亚卵形细胞增多症由红细胞带 3 基因中的 27-bp 缺失定义”《东南亚热带医学与公共卫生杂志》。

Takao,S.,Ishida,T. et al.: "Seroprevalence of human T-lymphotropic virus type 1 in Popma New Guinea and Irian Jaya measured using different western blot criteria"Journal of Clinical Virology. 16. 129-133 (2000)

高雄，S.，石田，T.