Genome stability and primate lifespan

为了支持人类更长的寿命，基因组的稳定性和大提琴的长复制寿命是不可或缺的条件。在动物园和猎人的合作下，收集并培养了来自各种哺乳动物物种的细胞，包括灵长类、食肉目、偶蹄目、长鼻目、兔形目、无齿目和翼手目。共储存了来自30个物种的70个细胞谱系。我们改进了培养技术，使有袋动物的细胞在不使用特殊培养基的情况下，用常规培养基培养也能存活。此外，培养体系的改进也使初学者取得了良好的成绩。细胞的复制寿命因物种而异;袋鼠、蝙蝠和熊表现出相对较大的PDL。P53是维持基因组和细胞周期的重要蛋白。我们比较了几种灵长类动物的氨基酸序列，发现包括人类在内的大型猿类和小型猿类之间富含脯氨酸的结构不同。这些活细胞现在储存在液氮中，将是一种有用的生物资源。

To support the longer lifespan of humans, stability of the genome and the long replicative lifespan of the cello are indispensable conditions. To investigate backgrounds for these conditions, culturable cell stocks are important.Under the cooperation of zoos and hunters, cells from various mammalian species including Primates, Carnivora, Artiodactyla, Proboscidea, Lagomorpha, Edentata and Chiroptera, were collected and cultured. A total of 70 cell lineages from 30 species were stored. We have improved the culture technology, and the cells of the marsupials were viable without a special medium but with conventional culture media. Moreover, the amelioration of the culture systems gave a good achievement to the beginners. The replicative lifespan of the cells varied by the species; kangaroos, bats and bears showed relatively large PDLs. P53 is an important protein in the maintenance of the genome and cell cycle. The amino acid sequence was compared between several primate species and we have found that the proline-rich structure is different between the great apes including humans and the lesser apes.These viable cells are now stored in liquid nitrogen and will be a useful biological resource.