Genome stability and primate lifespan

基因组稳定性和灵长类动物寿命

• 批准号: 18370097
• 负责人: ISHIDA Takafumi
• 金额: $ 10.59万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18370097/
• 关键词: Primates; Evolution; Hominization; Cell; lifespan; Genome; 比較ゲノム; 染色体

To support the longer lifespan of humans, stability of the genome and the long replicative lifespan of the cello are indispensable conditions. To investigate backgrounds for these conditions, culturable cell stocks are important.Under the cooperation of zoos and hunters, cells from various mammalian species including Primates, Carnivora, Artiodactyla, Proboscidea, Lagomorpha, Edentata and Chiroptera, were collected and cultured. A total of 70 cell lineages from 30 species were stored. We have improved the culture technology, and the cells of the marsupials were viable without a special medium but with conventional culture media. Moreover, the amelioration of the culture systems gave a good achievement to the beginners. The replicative lifespan of the cells varied by the species; kangaroos, bats and bears showed relatively large PDLs. P53 is an important protein in the maintenance of the genome and cell cycle. The amino acid sequence was compared between several primate species and we have found that the proline-rich structure is different between the great apes including humans and the lesser apes.These viable cells are now stored in liquid nitrogen and will be a useful biological resource.

为了支持人类更长的寿命，基因组的稳定性和大提琴的长复制寿命是必不可少的条件。在动物园和猎人的合作下，收集和培养了包括灵长类、食肉目、偶蹄目、鼻目、兔形目、伊顿目和翼手目在内的各种哺乳动物的细胞。共储存了30个物种的70个细胞谱系。我们对培养技术进行了改进，在没有特殊的培养液的情况下，有袋动物的细胞在常规培养基下仍能存活。此外，文化体系的完善给初学者带来了良好的成绩。细胞的复制寿命因物种而异；袋鼠、蝙蝠和熊的PDL相对较大。P53是维持基因组和细胞周期的重要蛋白质。对几个灵长类物种的氨基酸序列进行了比较，我们发现包括人类在内的大型类人猿和小型类人猿的富含脯氨酸的结构是不同的。这些活细胞现在储存在液氮中，将成为一种有用的生物资源。

项目成果

Ovalocytosis without band 3 gene 27-bp deletion and malaria infection

• DOI: 10.1537/ase.050802
• 发表时间: 2006-08-01
• 期刊: ANTHROPOLOGICAL SCIENCE
• 影响因子: 0.7
• 作者: Kimura, Masako;Soemantri, Augustinus;Ishida, Takafumi
• 通讯作者: Ishida, Takafumi

TP53 codon 72 polymorphism in 12 populations of insular Southeast Asia and Oceania

东南亚岛屿和大洋洲 12 个人群的 TP53 密码子 72 多态性

• 发表时间: 2007
• 期刊: Journal of Human Genetics 52
• 作者: Kashima;T.;et. al.
• 通讯作者: et. al.

Coexistence of five G6PD variants indicates ethnic complexity of Phuket islanders, Southern Thailand

• DOI: 10.1007/s10038-006-0380-y
• 发表时间: 2006-01-01
• 期刊: JOURNAL OF HUMAN GENETICS
• 影响因子: 3.5
• 作者: Ninokata, Aya;Kimura, Ryosuke;Ishida, Takafumi
• 通讯作者: Ishida, Takafumi

Quantitative analysis of cell-free Epstein-Barr virus DNA in the plasma of patients with peripheral T-cell and NK-cell lymphomas and peripheral T-cell proliferative diseases

外周 T 细胞和 NK 细胞淋巴瘤及外周 T 细胞增殖性疾病患者血浆中游离 Epstein-Barr 病毒 DNA 的定量分析

• 发表时间: 2007
• 期刊: Journal of Clinical Virology 40
• 作者: Suwiwat;S.;Pradutkanchana;J.;Ishida;T.;Mitarnun;W.
• 通讯作者: W.

Analysis of LMP1 variants of EBV in southern Thailand: Evidence for strain-associated T-cell tropism and pathogenicity

• DOI: 10.1016/j.jcv.2006.01.018
• 发表时间: 2006-06-01
• 期刊: JOURNAL OF CLINICAL VIROLOGY
• 影响因子: 8.8
• 作者: Saechan, Vannarat;Mori, Atsumi;Ishida, Takafumi
• 通讯作者: Ishida, Takafumi