The molecular epidemiological analyses of G6PD deficiency in Vietnam

越南G6PD缺乏症的分子流行病学分析

• 批准号: 12576029
• 负责人: NISHIYAMA Kaoru
• 金额: $ 9.73万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12576029/
• 关键词: G6PD deficiency; Mass screening; Vietnam; Genetic analysis; スクリーニング; ホルマザン法; 赤血球

The cooperative research of molecular epidemiological analyses of G6PD deficiency in Vietnam between Dao's group and our group has been developed. Within 3 years of this project, we could collect the dried blood samples (5,899) from newborn babies in Hanoi and screen them by our Formazan method. The positive incidence of G6PD deficiency was 3.1 %. This value was nearly same in Filipinos. However, its value from Hor Binh Province near Hanoi was 18 % and especially from Muong nations in Hor Binh, 32 %.From northern (Hanoi, Hoa Binh), central (Khanh Hoa, Hue) and southern Vietnam (Ho Chi Minh City) we could collect 137 samples for genomic analyses by MPTP method with their informed consent. Those contained Kinh, Muong, Katu, Dao, Raclei and Tay nations. Seven different variations such as Gaoha, Chatham, Chinese 5, Viangchan, Union, Canton and Kaiping could be identified. Union was major in Muong and Viangchan in Katu. These variants were popular in neighboring countries of Indonesia, Malaysia, Singapore, Philippines and Thailand. But from Vietnamese we could hardly obtain the variants of Mahidol, Mediterranean and Vanua Lava.

本课组与Dao课组开展了越南G6PD缺乏症分子流行病学分析的合作研究。在这个项目的三年内，我们可以从河内的新生儿中收集5899份干血样本，并通过我们的Formazan方法进行筛选。G6PD缺乏症阳性发生率为3.1%。菲律宾人的这一数值几乎相同。然而，来自河内附近的河平省的价值为18%，特别是来自河平的Muong民族的价值为32%。我们从越南北部（河内、和平）、中部（庆化、顺化）和南部（胡志明市）收集了137份样本，并征得他们的知情同意，采用MPTP方法进行基因组分析。这些国家包括京族、孟族、加图族、道族、腊族和德族。可以识别出高哈、查塔姆、中国5、Viangchan、Union、Canton和开平等七种不同的变体。联盟主要在蒙和在加图的Viangchan。这些变种在邻国印度尼西亚、马来西亚、新加坡、菲律宾和泰国很受欢迎。但是从越南语中我们很难得到Mahidol， Mediterranean和Vanua Lava的变体。

项目成果

Tran Thi Chinh et al.: "G6PD deficiency and HLA class II of Muong and Kinh ethnics in Hoa Binh and Hanoi-Viet nam"1er Colloque international francophone sur L-Histocompatibilite au Viet nam, Universite de Medicine Hanoi. I-XIV-9-XIV (2002)

Tran Thi Chinh 等人：“越南和平和河内 Muong 族和 Kinh 族的 G6PD 缺乏症和 HLA II 类”1er Colloque International francophone sur L-Histocompatibilite au Viet nam，河内医科大学。

Yusoff N.M., Shirakawa T., Nishiyama K., Ghazali S.et al.: "Molecular heterogeneity of glucose-6-phosphate dehydrogenase deficiency in Malays in Malaysia"Intern.J.Heamarol.. 76. 149-152 (2002)

Yusoff N.M.、Shirakawa T.、Nishiyama K.、Ghazali S.等人：“马来西亚马来人葡萄糖-6-磷酸脱氢酶缺乏症的分子异质性”Intern.J.Heamarol.. 76. 149-152 (2002)

Chinh T.T., An V.T., Khoa P.V., Dao N.T.N., et al.: "G6P deficiency and HLA class II of Muong and Kinh ethnics in Hoa Binh and Hanoi -Vietnam"1er Colloque international fracophone sur L-Histocompatibleite as Vietnam. 1-XiIV-1-XIV (2002)

Chinh T.T.、An V.T.、Khoa P.V.、Dao N.T.N. 等人：“越南和平和河内 Muong 族和 Kinh 族的 G6P 缺乏症和 HLA II 类”1er Colloque International fracophone sur L-Histocompatible as Vietnam。

Soweono T., Martin Shirakawa T., Nishyama K.: "Glucose 6 phosphate dehydrogenase(G6PD) deficiency variants in small isolated islands in eastern Inonesia"J.Kedokteran Yarsi.. 8. 87-92 (2000)

Soweono T.、Martin Shirakawa T.、Nishyama K.：“印度尼西亚东部小孤岛的葡萄糖 6 磷酸脱氢酶 (G6PD) 缺乏变异”J.Kedokteran Yarsi.. 8. 87-92 (2000)

Padilla C., Nishiyama K., Shirakawa T., Matsuo M.: "Screening for glucose-6-phosphate dehydrogenase deficiency using a method formazan method"Pediatrics International. 45. 10-15 (2003)

Padilla C.、Nishiyama K.、Shirakawa T.、Matsuo M.：“使用甲臜法筛选葡萄糖-6-磷酸脱氢酶缺乏症”国际儿科。