Study on detection of urinary spermine in cadaver

在我们的兴奋剂测试经验中，我们发现检测到多胺。尿中精胺的检测在细胞生长中起着非常重要的作用，如果精胺的释放影响了任何死亡途径，有时会在相当高的水平上引起我们的兴趣。在这项研究中，一个快速和。采用气相色谱-质谱法(GC/MS)建立了尿液中多胺的准确定量分析方法。采用高效液相色谱-柱后衍生荧光检测和GC/MS联用技术，对法医身体和急危重症医院患者尿中精胺浓度进行了初步研究。用市售氨基酸分析系统进行高效液相色谱分析，用我们的苯丙胺分析方法改进GC/MS，通过填充硅藻土的萃取管进行萃取衍生化(七氟丁酰化)。男性身体的平均尿精胺浓度明显高于女性身体。在身体和患者的尿液精胺水平比较中，身体的水平相对较高。为方便起见，阳性标准的阈值设置为身体为80nmoL/毫升，患者为-20nmoL/毫升。高分尿精胺仅见于有心脏病、脑损伤和肝损伤的男性身体，以及主要由心功能不全、脑损伤等引起的成人呼吸骤停综合征的男性患者，提示男性生前释放精胺有一定的机制。

In our experience of stimulant drug test, we found detecting polyamines. Urinary detection of spermine, which plays very important roles in cell growth, occasionally in considerably high level attracted in our interest if the release of spermine affected any pathway of death. In this study, a rapid and. accurate quantitative analytical method for urinary polyamines was developed using gas chromatography/mass spectrometry (GC/MS). By using high performance liquid chromatography with post-column derivatization on fluorescence detection (HPLC) and GC/MS, urinary spermine concentrations of cadavers in forensic medicine and patients in emergency and critical care hospital were measured for preliminary study. HPLC was performed by using amino acid analytical system commercially provided, and GC/MS was developed modifying with our amphetamine assay in which an extractive derivatization (heptafluorobutyrylation) was carried out in passing through an extracting tube filled with diatomaceous earth. Average of urinary spermine concentrations were considerably higher in male cadavers than in female ones. In comparison of cadavers and patients in urinary spermine levels, cadaver levels were relatively higher. Threshold levels for positive criterion were, for convenience sake, set at 80 nmol/mL for cadavers and-at 20 nmol/mL for patients. High scores of urinary spermine were only indicated in male cadavers with heart diseases, brain injuries and liver injuries, and in male patients mainly with adult arrest respiratory disease syndrome due to cardiac dysfunction, brain injuries, etc. These results suggest that there are some mechanisms in man releasing spermine before death.