Study on detection of urinary spermine in cadaver

在兴奋剂检测中，我们发现了多胺的检测。精胺在细胞生长中起着非常重要的作用，如果精胺的释放影响任何死亡途径，偶尔会在相当高的水平上引起我们的兴趣。在这项研究中，快速和。建立了尿中多胺的气相色谱/质谱（GC/MS）定量分析方法。采用高效液相色谱-柱后衍生荧光检测法（HPLC）和气相色谱-质谱联用技术（GC/MS），对法医尸体和急危重症患者尿精胺浓度进行了初步研究。通过使用商业上提供的氨基酸分析系统进行HPLC，并且开发了GC/MS，其修改了我们的安非他明测定，其中在通过填充有硅藻土的提取管的过程中进行提取衍生化（七氟丁酰化）。男性尿精胺浓度明显高于女性。在尿精胺水平的尸体和病人相比，尸体水平相对较高。为方便起见，将阳性标准的阈值水平设定为80 nmol/mL（尸体）和20 nmol/mL（患者）。高评分的尿精胺仅见于心脏病、脑损伤和肝损伤的男性尸体，而男性患者主要是由于心功能不全、脑损伤等引起的成人呼吸道疾病综合征。这些结果提示，人死前有一定的精胺释放机制。

In our experience of stimulant drug test, we found detecting polyamines. Urinary detection of spermine, which plays very important roles in cell growth, occasionally in considerably high level attracted in our interest if the release of spermine affected any pathway of death. In this study, a rapid and. accurate quantitative analytical method for urinary polyamines was developed using gas chromatography/mass spectrometry (GC/MS). By using high performance liquid chromatography with post-column derivatization on fluorescence detection (HPLC) and GC/MS, urinary spermine concentrations of cadavers in forensic medicine and patients in emergency and critical care hospital were measured for preliminary study. HPLC was performed by using amino acid analytical system commercially provided, and GC/MS was developed modifying with our amphetamine assay in which an extractive derivatization (heptafluorobutyrylation) was carried out in passing through an extracting tube filled with diatomaceous earth. Average of urinary spermine concentrations were considerably higher in male cadavers than in female ones. In comparison of cadavers and patients in urinary spermine levels, cadaver levels were relatively higher. Threshold levels for positive criterion were, for convenience sake, set at 80 nmol/mL for cadavers and-at 20 nmol/mL for patients. High scores of urinary spermine were only indicated in male cadavers with heart diseases, brain injuries and liver injuries, and in male patients mainly with adult arrest respiratory disease syndrome due to cardiac dysfunction, brain injuries, etc. These results suggest that there are some mechanisms in man releasing spermine before death.