Preliminary study on endogenous disease diagnosis by chemical analysis in hair

有些疾病是由代谢紊乱引起的。因此，生化信息将在疾病中发挥重要作用。目前还没有足够的报告可以通过生化信息来诊断这些疾病。为了获得生化信息，尿液和血液在临床诊断中是有用的。然而，样本应该来自在测试中控制饮食的患者。因此，身体标本不适合用于这一目的。同时，据说毛发药物分析是展示慢性药物治疗(或使用)的有效工具。收集的毛发可以很容易地在环境条件下保存。我们的研究项目是基于头发药物测试的计划，如果多余的生物化合物被头发保留。1.采用气相色谱-质谱联用技术，结合Extrelut[0！R]色谱柱上的七氟正丁酰化萃取物提取毛发样品中的化学信息。该方法对低浓度的胺、氨基酚类物质有较高的灵敏度。2.毛发中明显检测到三种化合物。在其中两个(未鉴定的)独立样本之间没有定量观察到的差异。酪胺(其中一种)的检测被定量地分为两组。3.除腐烂标本外，约30%的标本毛发中含有较高水平的酪胺。观察了相对数量的苯丙胺滥用者、心脏病患者和精神分裂症患者的头发样本。通过这一项目工作，指出了毛发分析在解决迄今尚未被掩盖的代谢障碍方面的未来用途。

Some disease is caused by metabolic disorders. Therefore, biochemical information would play an important role for disorders. There are no adequate reports to diagnose those disorders by biochemical information. To obtain biochemical information, urine and blood are useful in clinical diagnosis. However, the samples should be taken from patients who are diet-controlled for the test. So that, postmortem samples are not suitable for the purpose. Meanwhile, it is said that hair drug analysis is an effective tool for demonstrating chronic drug therapy (or use). Hair collected can be easily preserved under ambient conditions. Our research project was planned based on the hair drug test, if excess biogenic compounds were retained by hair. 1. Gas chromatographic-mass spectrometric analysis together with extractive heptafluoro-n-butyrylation on Extrelut【○!R】 column was employed for acquiring chemical information from hair specimens. In this method, amines, aminophenols were sensitively detected in low level. 2. There were three compounds remarkably detected in hair. No difference among independent, specimens was quantitatively observed in two of them (unidentified). The detections of tyramin (one of them) were quantitatively divided into two different groups. 3. High levels of tyramine in hair were observed in about 30% of total specimens excepting putrefied specimens. Relatively numbers of hair specimens obtained from amphetamine-abusers, heart-disease holders and schizophrenic were observed. By this project work, the future utility of hair analysis is pointed out in resolving metabolic disorders that have so far been veiled.