Biological analyses of articular disk of temporomandibular joint and the adjacent tissues during growth and senescence.

颞下颌关节关节盘及邻近组织生长和衰老过程的生物学分析。

• 批准号: 13470425
• 负责人: SATO Hironobu
• 金额: $ 7.49万
• 依托单位: Fukuoka Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13470425/
• 关键词: osteoarthritis; proteoglycan; glycosaminoglycan; cartilage; bone marrow; protein distribution; gene expression; temporomandibular joint; デコリン; バイグリカン; フィブロモデリン; ルミカン; 老化促進マウス; 老化促進モデルマウス; 関節軟骨; コンドロイチン4硫酸; ケラタン硫酸; 膝関節; 骨粗鬆症; 成長板軟骨; 加齢

Molecular basis of onset of mandibular osteoarthritis (OA) has not been elucidated. It is believed that collagens and proteoglycans have crucial structural roles in maintenance of epiphyseal cartilage and subchondral bone and in process of OA at the other bones. Among proteoglycans, collagen-binding small leucine rich proteoglycans (SLRPs) which regulate formation of collagen fibrils may be involved in the onset and the process of mandibular OA. To test this hypothesis, we investigate distribution of the glycosaminoglycans (GAGs) of SLRPs in mandibular condylar cartilage with an early stage of OA and expression of SLRP genes in mandibular subchondral bone marrow before the onset of OA using senescence-accelerated mice (SAM). Chondroitin-4 sulphate (C4S) and keratin sulphate (KS) had a different distribution ; the former was distributed throughout all layers of the cartilage but the latter was exclusively in the surface of cartilage, fibrous and proliferative cell layers. Once fissures appeared in the cartilage, the two GAGs was, however, strongly located around the fissures, suggesting that C4S and KS may have a role in degenerative cartilage of mandibular OA and that their structural functions possibly compensate those of degenerated collagen networks. In the mandibular subchondral bone marrows, mRNA expression levels of collagen-binding SLRPs, decorin, biglycan, fibromodulin, and lumican, were equivalent between SAMP8 with mandibular OA and SAMR1 with no degenerative mandibular condyles. In the femoral subchondral bone marrows, biglycan was expressed in SAMP8 but not in SAMR1, possibly implying that SAMP8 with mandibular OA phenotype has different bone quality compared with SAMR1. Collectively, although more molecular biological and biochemical analyses are needed, collagen-binding SLRPs may be involved in the structural functions of cartilage and bone and in the onset and the proceeding of mandibular OA.

下颌骨骨关节炎（OA）发病的分子基础尚未阐明。据信胶原和蛋白多糖在骨骺软骨和软骨下骨的维持以及其他骨的OA过程中具有关键的结构作用。在蛋白多糖中，胶原结合小亮氨酸蛋白多糖（SLRP）调节胶原纤维的形成，可能参与了下颌骨OA的发病和发展。为了验证这一假设，我们调查的糖胺聚糖（GAGs）的SLRP在下颌骨髁突软骨骨关节炎的早期阶段和表达的SLRP基因在下颌骨软骨下骨髓OA发病前使用加速老化小鼠（SAM）。硫酸软骨素-4（C4 S）和硫酸角蛋白（KS）的分布不同，前者分布于软骨的所有层，而后者仅分布于软骨表面、纤维层和增殖细胞层。然而，一旦软骨中出现裂缝，两个GAG强烈地位于裂缝周围，这表明C4 S和KS可能在下颌骨OA的退行性软骨中起作用，并且它们的结构功能可能补偿退行性胶原网络的功能。在下颌骨软骨下骨髓中，患有下颌骨OA的SAMP 8和没有退行性下颌骨髁突的SAMR 1之间的胶原结合SLRP、核心蛋白聚糖、双糖蛋白聚糖、纤维调蛋白和Lumican的mRNA表达水平相当。在股骨软骨下骨髓中，双糖蛋白聚糖在SAMP 8中表达，而在SAMR 1中不表达，这可能意味着具有下颌骨OA表型的SAMP 8与SAMR 1相比具有不同的骨质量。总的来说，虽然需要更多的分子生物学和生化分析，胶原结合SLRP可能参与软骨和骨的结构功能，并在下颌骨OA的发病和进行。

项目成果

S.Kuramoto, T.Matsuura, T.Tsuzuki, H.Sato: "Distribution of glycosaminoglycans in an early osteoarthritis-like lesion in the mandibular condylar cartilage of senescence-accelerated mice."Oral Medicine and Pathology. In press. (2004)

S.Kuramoto、T.Matsuura、T.Tsuzuki、H.Sato：“衰老加速小鼠下颌髁软骨中早期骨关节炎样病变中糖胺聚糖的分布。”口腔医学和病理学。

都築尊, 松浦尚志, 松隈敬, 蔵本茂禎, 今村英之, 石川昌嗣, 佐藤博信: "マウス下顎頭における成長に伴うコラーゲンの局在変化"日本顎関節学会総会・学術大会(プログラム・抄録集). 14. 68-68 (2001)

Takashi Tsuzuki、Takashi Matsuura、Takashi Matsukuma、Shigesada Kuramoto、Hideyuki Imamura、Masashi Ishikawa、Hironobu Sato：“小鼠下颌髁突生长中胶原蛋白的局部变化”日本颞下颌关节学会会员大会和学术会议（议程/摘要集） ) 14. 68-68 (2001)。

蔵本茂禎, 都築尊, 羽生真也, 後藤洋介, 今村英之, 松浦尚志 佐藤博信: "変形性顎関節症の発症メカニズムに関する研究-グリコサミノグリカン鎖の局在-"日本補綴歯科学会雑誌. 46. 138-138 (2002)

Shigeta Kuramoto、Takashi Tsuzuki、Shinya Hanyu、Yosuke Goto、Hideyuki Imamura、Takashi Matsuura、Hironobu Sato：“颞下颌关节骨关节炎的发病机制研究 - 糖胺聚糖链的定位 -” 日本修复医学会杂志 46 .138-。 138（2002）

Nagashima Y, Matsuura T, Yamamoto K, Sato H, Matsuura M: "Higher expression of collagen-binding small leucine-rich proteoglycan genes in mouse mandibular bone marrow than femorol bone marrow."Journal of Hard Tissue Biology. 12. 56-62 (2003)

Nagashima Y、Matsuura T、Yamamoto K、Sato H、Matsuura M：“小鼠下颌骨髓中胶原蛋白结合的富含亮氨酸的小蛋白多糖基因的表达高于股骨骨髓。”硬组织生物学杂志。

佐藤博信, 松浦尚志: "退行性病変の画像診断,顎関節症(日本顎関節学会編)"永松書店. 5 (2003)

佐藤博信、松浦隆：“退行性病变、颞下颌关节疾病的影像诊断（日本颞下颌关节学会编）”长松书店5（2003）。