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Mechanism and prevention of periodontal pocket formation

牙周袋形成机制及预防

基本信息

批准号：
13470450
负责人：
WATANABE Tatsuo
金额：
$ 9.02万
依托单位：
OKAYAMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2004
项目状态：
已结题

项目摘要

The purposes of this research project were to establish rat model of periodontal pocket formation, to clarifymechanism of pocket formation, and to evaluate effects of mechanical stimulation of gingiva on healing of periodontal pocket.In the year 2001, rat model of periodontal pocket formation was established with application of lipopolysaccharide and bacterial proteases into gingival sulcus. Apical migration of junctional epithelium along the root surface and apoptosis of periodontal ligament fibroblast were followed by promoted proliferative activity of junctional epithelium and collagen destruction at the initiation of periodontal pocket formation.In the year 2002, optimal duration of mechanical stimulation to promote cell proliferation in normal gingiva was determined. Optimal duration of mechanical stimulation to promote proliferation of junctional epithelial cells and fibroblasts were 10 or 20 seconds and 5 or 10 seconds, respectively.In the year 2003, effects of mechanical stimulation on healing of periodontal pocket were evaluated using the rat pocket formation model established in the year 2001 and duration of stimulation determined in the year 2002. Mechanical stimulation reduced number of polymorphonuclear leukocytes in both junctional epithelium and connective tissue, and reduced area of destroyed collagen fibers.In the year 2004, effects of mechanical stimulation on cell cycle of normal gingiva were evaluated using proliferating cell nuclear antigen(PCNA) and bromodeoxyuridine(BrdU). Mechanical stimulation increased both numbers of PCNA- and BrdU-positive cells in junctional epithelial cells and fibroblasts. The results suggest that mechanical stimulation enhances DNA synthesis of junctional epithelial cells and fibroblasts.The results of this project contribute to develop effective periodontal treatment using activation of host defense by mechanical stimulation of gingiva.

本研究旨在建立大鼠牙周袋形成模型，探讨牙周袋形成的机制，评价机械刺激牙周袋对牙周袋愈合的影响。在牙周袋形成初期，牙周联合上皮沿牙根表面的顶端迁移和牙周膜成纤维细胞的凋亡，促进了联合上皮的增殖活性和胶原的破坏。机械刺激促进连接上皮细胞和成纤维细胞增殖的最佳时间分别为10或20秒和5或10秒。2003年，利用2001年建立的大鼠牙周袋形成模型和2002年确定的刺激时间，评价了机械刺激对牙周袋愈合的影响。2004年，用增殖细胞核抗原(PCNA)和溴脱氧尿嘧啶核苷(BrdU)法检测了机械刺激对正常牙周组织细胞周期的影响。机械刺激使连接上皮细胞和成纤维细胞中的增殖细胞核抗原阳性细胞和BrdU阳性细胞数均增加。研究结果提示，机械刺激可促进牙周连接上皮细胞和成纤维细胞的DNA合成。本项目的研究结果有助于开发有效的牙周治疗方法，即通过机械刺激牙龈来激活宿主防御。