Absorption Kinetics of the Main Allergen in Egg White on the Intestinal Mucous Membrane of Infants

本研究调查了以下几个方面：1.过敏大鼠（视为婴儿）中过敏原通过肠粘膜（IMM）进入蛋清（蛋白质）2.食物过敏发生后IMM的状况3.过敏大鼠通过给予过敏原食物诱导免疫耐受获得以下结果：1.在幼年大鼠中，发生过敏后通过IMM传递的过敏原数量明显多于之前。此外，过敏发生两周后通过 IMM 的过敏原数量明显多于刚刚发生后的数量。因此，过敏反应是在过敏发生后1-2周内介导到IMM上的。2.给予过敏性食物22天后，观察到皮肤出现过敏性炎症。 IMM 上的派尔氏斑扩大并肿胀。过敏原给药组观察到杯状细胞和绒毛泡崩解； 3.免疫耐受方面，连续给予过敏原后，IL-4和IFN-γ水平分别降低和升高。在间断组中，IL-4水平在过敏原施用的第二周升高，此时施用已经停止。当施用过敏原后不久停止施用时，脾细胞中的T细胞主要分化并增殖为Th2细胞。然而，当再次施用过敏原时，诱导T细胞分化为Th1细胞，并且观察到总IgE或OM IgE抗体单位显着减少，表明诱导了免疫耐受。这里，建议以不引起过敏的适当剂量口服施用过敏原是有效的由于其免疫或免疫反应降低而进行过敏治疗。

This study investigated the following aspects :1.Passage of allergens into the egg white (protein) via the intestinal mucous membrane (IMM) in allergic rats (considered as infants)2.Condition of the IMM after development of food allergy3.Immune tolerance induction by the administration of allergen foods in allergic ratsThe following results were obtained :1.In juvenile rats, a significantly larger amount of allergens passed via the IMM after development of allergy than before. Moreover, the amount of allergens passing via the IMM two weeks after allergy development was significantly larger than that immediately after. Therefore, the allergic effect was mediated to the IMM within one-two weeks of allergy development.2.Allergic inflammation was observed on the skin 22 days after the administration of allergic food. Peyer's patches on the IMM were expanded and swollen. Disintegration of goblet cells and villous blebs was observed in the allergen administration group ; the mast cell number increased significantly in the jejunum tissues.3.Regarding immune tolerance, the IL-4 and IFN-γ levels decreased and increased, respectively, after continuous allergen administration. In the discontinuous group, the IL-4 level increased in the second week of allergen administration when administration had already been discontinued. When the allergen administration was discontinued shortly after its administration, the T-cells in the splenic cells mainly differentiated and proliferated into Th2 cells.However, when allergen administration was resumed, differentiation of T cells into Th1 cells was induced, and a significant decrease was observed in the total IgE or OM IgE antibody units, suggesting the induction of immune tolerance.Here, oral administration of the allergen in an appropriate dose that did not cause allergy was suggested to be effective in allergy treatment because of its decreased immunization or immune response.