Absorption Kinetics of the Main Allergen in Egg White on the Intestinal Mucous Membrane of Infants

蛋清中主要过敏原在婴儿肠粘膜上的吸收动力学

• 批准号: 15500570
• 负责人: TAKAHASHI Kyoko
• 金额: $ 2.05万
• 依托单位: Mukogawa Women's University Junior College Division
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15500570/
• 关键词: Food allergy; Allergen in egg white; Oral tolerance; Mucosa of small intestine; Ovomucoid; Mucous membrane organization; Cytokine; Antibody titer; s IgA; sIgA

This study investigated the following aspects :1.Passage of allergens into the egg white (protein) via the intestinal mucous membrane (IMM) in allergic rats (considered as infants)2.Condition of the IMM after development of food allergy3.Immune tolerance induction by the administration of allergen foods in allergic ratsThe following results were obtained :1.In juvenile rats, a significantly larger amount of allergens passed via the IMM after development of allergy than before. Moreover, the amount of allergens passing via the IMM two weeks after allergy development was significantly larger than that immediately after. Therefore, the allergic effect was mediated to the IMM within one-two weeks of allergy development.2.Allergic inflammation was observed on the skin 22 days after the administration of allergic food. Peyer's patches on the IMM were expanded and swollen. Disintegration of goblet cells and villous blebs was observed in the allergen administration group ; the mast cell number increased significantly in the jejunum tissues.3.Regarding immune tolerance, the IL-4 and IFN-γ levels decreased and increased, respectively, after continuous allergen administration. In the discontinuous group, the IL-4 level increased in the second week of allergen administration when administration had already been discontinued. When the allergen administration was discontinued shortly after its administration, the T-cells in the splenic cells mainly differentiated and proliferated into Th2 cells.However, when allergen administration was resumed, differentiation of T cells into Th1 cells was induced, and a significant decrease was observed in the total IgE or OM IgE antibody units, suggesting the induction of immune tolerance.Here, oral administration of the allergen in an appropriate dose that did not cause allergy was suggested to be effective in allergy treatment because of its decreased immunization or immune response.

本研究探讨了以下几个方面：1.过敏大鼠（幼鼠）过敏原经肠粘膜（IMM）进入白色（蛋白）的途径; 2.食物过敏后IMM的状况; 3.过敏大鼠给予过敏原食物后的免疫耐受诱导。此外，过敏发生后两周通过IMM的过敏原的量显著大于之后立即通过IMM的量。因此，在过敏发展的1 - 2周内，过敏效应是由IMM介导的。2.在给予过敏性食物后22天，在皮肤上观察到过敏性炎症。IMM上的派伊尔集合淋巴结扩张肿胀。致敏组空肠黏膜杯状细胞和绒毛泡崩解，肥大细胞数量明显增多。3.免疫耐受方面，持续致敏后IL-4和IFN-γ水平分别下降和升高。在间断组中，IL-4水平在变应原给药的第二周增加，此时已经停止给药。当在给药后不久停止给药时，脾细胞中的T细胞主要分化和增殖为Th 2细胞。然而，当恢复给药时，T细胞被诱导分化为Th 1细胞，并且观察到总IgE或OM IgE抗体单位显著降低，这表明诱导了免疫耐受。以不引起变态反应的适当剂量口服施用过敏原被认为在变态反应治疗中是有效的，因为其降低了免疫或免疫应答。

项目成果

Determination of ovomucoid as allergen in egg contain process foods. - Comparison between amount of ovomucoid to monoclonal antibody and allergen to patient serum. -

含蛋加工食品中作为过敏原的卵类粘蛋白的测定。

• 发表时间: 2004
• 期刊: New Food Industry 46(4)
• 作者: Yoko Kamada;Kazuyo Ohmuro;Masuharu Hattori;Kyoko Takahashi
• 通讯作者: Kyoko Takahashi

卵含有加工食品中のアレルゲン・オボムコイド定量 -モノクローナル抗体に対するオボムコイド量と患者血清に対するアレルゲン量の比較-

含鸡蛋加工食品中过敏原和卵类粘蛋白的定量 - 卵类粘蛋白水平与单克隆抗体的比较以及过敏原水平与患者血清的比较 -

• 发表时间: 2004
• 期刊: New Food Industry 46(4)
• 作者: 鎌田 陽子;大室 和代;服部 益治;高橋 享子
• 通讯作者: 高橋 享子