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Analysis of Ewing sarcoma using a transgenic mouse

Analysis of Ewing sarcoma using a transgenic mouse
使用转基因小鼠分析尤文肉瘤
批准号:
15591593
负责人:
YOSHIDA Koichi
金额:
$2.37万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2003
资助国家:
日本
项目状态:
已结题
起止时间:
2003 至 2004

项目摘要

项目成果

YOSHIDA Koichi的其他基金

相关文献

中文摘要
翻译
本研究的目的是在尤因肉瘤发生的母体组织中表达癌基因,并分离实验小鼠模型。进一步,确定EWS-FLI融合转录因子控制的靶基因。我们分离了两株携带EWS-FLI癌基因的转基因小鼠。这些小鼠具有生殖潜能,能够在正常条件下繁殖。我们将这些小鼠与神经嵴表达Cre酶的实验小鼠杂交。这使我们能够在体内诱导DNA重组,并在神经嵴源性细胞中激活EWS-FLI致癌基因。由于获得的小鼠数量不够,我们无法判断肉瘤的发生。我们将分离出一种不同的转基因小鼠,并与Cre转基因小鼠再次杂交。在我们引入致癌基因EWS-FLI的细胞系中,我们发现该基因的表达发生了显著改变。通过cDNA阵列分析,我们发现EWS-FLI激活了细胞外基质成分tenascin基因。我们还发现一个融合转录因子激活了与细胞永生相关的端粒酶基因。我们将继续制作Ewing肉瘤模型实验小鼠,并将研究靶基因在肿瘤发展中的作用。
英文摘要
A goal of this study is to express the oncogene in mother tissue for Ewing sarcoma-genesis and to isolate a model laboratory mouse. Furthermore, it is to identify a target gene which the EWS-FLI fusion transcription factor controls. We isolated a transgenic mouse of two strains carrying EWS-FLI oncogene. These mice had reproductive potential and were able to propagate in a normal condition. We crossbred these mice with the laboratory mouse which expresses Cre enzyme in neural crest. This allows us to induce a recombination of DNA in vivo and an activation of EWS-FLI oncogene in neural crest-derived cells. Since mouse was not obtained in enough numbers, we were unable to judge sarcoma-genesis. We are going to isolate a different strain of transgenic mouse and crossbreed it with the Cre transgenic mouse again. In the cell line which we introduced the oncogene EWS-FLI into, we found the gene which expression was significantly altered. We made clear by cDNA array method that the tenascin gene, an extra-cellular matrix component, was activated by EWS-FLI. Also we found that a fusion transcription factor activated telomerase gene which is associated with cellular immortalization. We continue manufacture of model laboratory mouse of Ewing sarcoma and are going to examine a role of target gene in development of the neoplasm.
期刊论文(28)
专著(0)
科研奖励(0)
会议论文
Emi Ito: "A tetraspanin-family protein, T-cell acute lymphoblastic leukemia-associated Antigen 1, is induced by the Ewing's sarcoma-Wilms' Tumor 1"American Journal of Pathology. 163・6. 2165-2172 (2003)
Emi Ito:“四跨膜蛋白家族蛋白,T 细胞急性淋巴细胞白血病相关抗原 1,由尤文氏肉瘤 - 维尔姆斯肿瘤 1 诱导”美国病理学杂志 163·6 (2003)。
DOI: --
发表时间:
期刊:
影响因子: --
作者: []
通讯作者:
DOI: 10.1002/gcc.10153
发表时间: 2003-03
期刊: Genes
影响因子: 3.5
作者: [G. Watanabe;H. Nishimori;H. Irifune;Y. Sasaki;S. Ishida;H. Zembutsu;Toshihiro Tanaka;S. Kawaguchi;T. Wada;J. Hata;M. Kusakabe;Koichi Yoshida;Yusuke Nakamura;T. Tokino]
通讯作者: G. Watanabe;H. Nishimori;H. Irifune;Y. Sasaki;S. Ishida;H. Zembutsu;Toshihiro Tanaka;S. Kawaguchi;T. Wada;J. Hata;M. Kusakabe;Koichi Yoshida;Yusuke Nakamura;T. Tokino
E1AF/PEA3 reduces the invasiveness of SiHa cervical cancer cells by activating serine proteinase inhibitor squamous cell carcinoma antigen.
E1AF/PEA3通过激活丝氨酸蛋白酶抑制剂鳞状细胞癌抗原来降低SiHa宫颈癌细胞的侵袭性。
DOI: --
发表时间: 2004
期刊: Exp.Cell Res. 299
影响因子: --
作者: [Iwasaki, M.]
通讯作者: M.
EWS/ETS fusions activate teromerase in Ewing's tumors
EWS/ETS 融合激活尤文氏肿瘤中的端粒酶
DOI: --
发表时间: 2003
期刊: Cancer Research 63
影响因子: --
作者: [Iwasaki M, Nishikawa A, Akutagawa N, Fujimoyo T, Teramoto M, Sakaguchi Y, Kato H, Ito M, Yoshida K, Kudo R., Akiko Takahashi]
通讯作者: Akiko Takahashi
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