Research on gene copy number and resistance to EGFR-TKI therapy using silver in situ hybridization(SISH) technology for non-small-cell lung cancer

Ventana Medical Systems（Tucson， AZ）设计的实验性SISH探针用于评估10种非小细胞肺癌（NSCLC）细胞系的IGF1R基因拷贝数。IGF1R基因拷贝数/细胞核数平均为2.26（范围：1.12-7.56）。中位拷贝数为2.11。与非鳞状细胞癌相比，鳞状细胞癌的拷贝数为7.56。SISH检测的IGF1R基因拷贝数在鳞状细胞癌细胞系中高于其他非小细胞肺癌细胞系。

An experimental SISH probe designed by Ventana Medical Systems(Tucson, AZ) was used to evaluate IGF1R gene copy number on 10 non-small-cell lung cancer(NSCLC) cell lines. The mean number of IGF1R gene copies/nuclei per cell line was 2.26(range : 1.12-7.56). The median copy number was 2.11.Squamous cell carcinoma had 7.56 copy number compared to non-SCC histologies. IGF1R gene copy number detected by SISH is higher in Squamous cell carcinoma cell line than in other cell line of NSCLC.