Research on gene copy number and resistance to EGFR-TKI therapy using silver in situ hybridization(SISH) technology for non-small-cell lung cancer

利用银原位杂交（SISH）技术研究非小细胞肺癌基因拷贝数及EGFR-TKI治疗耐药

• 批准号: 22791323
• 负责人: YOSHIDA Koichi
• 金额: $ 2.08万
• 依托单位: Tokyo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22791323/
• 关键词: 肺癌; EGFR; EGFR-TKI; EGFR遺伝子変異; 上皮成長因子受容体; 分子標的治療; 薬剤耐性; IGF-1R; FISH; SISH; 耐性; IGF1R; 耐性因子; 化学療法; 遺伝子増幅

An experimental SISH probe designed by Ventana Medical Systems(Tucson, AZ) was used to evaluate IGF1R gene copy number on 10 non-small-cell lung cancer(NSCLC) cell lines. The mean number of IGF1R gene copies/nuclei per cell line was 2.26(range : 1.12-7.56). The median copy number was 2.11.Squamous cell carcinoma had 7.56 copy number compared to non-SCC histologies. IGF1R gene copy number detected by SISH is higher in Squamous cell carcinoma cell line than in other cell line of NSCLC.

Ventana Medical Systems（Tucson，AZ）设计的实验性SISH探针用于评估10种非小细胞肺癌（NSCLC）细胞系上的IGF 1 R基因拷贝数。每个细胞系的IGF 1 R基因拷贝/细胞核的平均数为2.26（范围：1.12-7.56）。中位拷贝数为2.11，鳞状细胞癌中拷贝数为7.56。SISH检测到的鳞状细胞癌细胞系中IGF 1 R基因拷贝数高于其他非小细胞肺癌细胞系。

项目成果

EGFR Protein Expression in Non-Small Cell Lung Cancer Predicts Response to an EGFR Tyrosine Kinase Inhibitor-A Novel Antibody for Immunohistochemistry or AQUA Technology

• DOI: 10.1158/1078-0432.ccr-11-0209
• 发表时间: 2011-12-15
• 期刊: CLINICAL CANCER RESEARCH
• 影响因子: 11.5
• 作者: Mascaux, Celine;Wynes, Murry W.;Hirsch, Fred R.
• 通讯作者: Hirsch, Fred R.

The role of IGF-1R in EGFR TKI resistance in NSCLC using IHC and AQUA technology

使用 IHC 和 AQUA 技术研究 IGF-1R 在 NSCLC EGFR TKI 耐药中的作用

• 发表时间: 2011
• 作者: Kato Y;Mascaux C;Wynes MW;Reyne Asuncion B;Tran C;Yoshida K;Matsubayashi J;Nakajima E;Ohira T;Nagao T;Furukawa K;Ikeda N;Hirsch FR
• 通讯作者: Hirsch FR

Novel epidermal growth factor receptor mutation-specific antibodies for non-small cell lung cancer: immunohistochemistry as a possible screening method for epidermal growth factor receptor mutations.

• DOI: 10.1097/jto.0b013e3181e9da60
• 发表时间: 2010-10
• 期刊: Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer
• 作者: Kato Y;Peled N;Wynes MW;Yoshida K;Pardo M;Mascaux C;Ohira T;Tsuboi M;Matsubayashi J;Nagao T;Ikeda N;Hirsch FR
• 通讯作者: Hirsch FR

Early experience using the da Vinci Surgical System for the treatment of mediastinal tumors

达芬奇手术系统治疗纵隔肿瘤的早期经验

• 发表时间: 2011
• 期刊: Gen Thorac Cardiovasc Surg
• 作者: Kajiwara N;Taira M;Yoshida K;Hagiwara M;Kakihana M;Usuda J;Uchida O;Ohira T;Kawate N;Ikeda N
• 通讯作者: Ikeda N

Prognostic Impact of Number of Resected and Involved Lymph Nodes at Complete Resection on Survival in Non-small Cell Lung Cancer

• DOI: 10.1097/jto.0b013e31822a35c3
• 发表时间: 2011-11-01
• 期刊: JOURNAL OF THORACIC ONCOLOGY
• 影响因子: 20.4
• 作者: Saji, Hisashi;Tsuboi, Masahiro;Ikeda, Norihiko
• 通讯作者: Ikeda, Norihiko