Development of drug delivery system for bone formation using cytokine containing DNA film

使用含有细胞因子的 DNA 膜开发骨形成药物输送系统

• 批准号: 15592091
• 负责人: INOUE Yusuke
• 金额: $ 2.18万
• 依托单位: Fukuoka College of Health Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15592091/
• 关键词: DNA Film; Cytokine; Bone Formation; DDS; Artificial lipid; Poly (L-lactine); Chitosan; DNA; ドラッグデリバリーシステム; BMP; FGF; 吸収性担体

The self-standing, water-insoluble DNA/lipid films containing cytokine were prepared to develop the Drug Delivery System (DDS) for bone formation. The results were as follows : The DNA/lipid films formed intercalation complexes with ethidium bromide. That indicated that the DNA/lipid films maintained a double helical structure. The tensile strength of DNA/lipid films were 0.8-2.4 MPa and were comparable with a commercial available Membrane for guided tissue regeneration in dental use. There were no inflammatory reactions or inhibition of new tissue formation. And also, the DNA/lipid films showed antibacterial and antifungal activities against bacteria or candida species. On the other hand, the DNA/lipid films were almost dissolved three days after implantation in the backs of rats regardless of lipids structure. Since the chitosan can bind easily to DNA, we chose the chitosan as a reagent combined with DNA instead of artificial lipids. The DNA/chitosan complexes were not toxic to MG-63 osteoblast-like cells and caused only a mild tissue response in the backs of rats. And also, the daunorubicin hydrochloride complex intercalated with DNA/chitosan complexes. On the other hand, the DNA/lipid/poly-lactic-acid films were also investigated. These films also maintained a double helical structure because the daunorubicin hydrochloride complex intercalated with DNA/lipid/poly-lactic-acid films. These results suggest the DNA/chitosan complexes or DNA/lipid/poly-lactic-acid films may be useful as DDS materials

制备了自支撑的、水不溶性的含细胞因子的DNA/脂质膜，以开发用于骨形成的药物递送系统（DDS）。结果表明：DNA/脂质膜与溴化乙锭形成嵌入复合物。这表明DNA/脂质膜保持了双螺旋结构。DNA/脂质膜的拉伸强度为0.8-2.4 MPa，与牙科用引导组织再生的市售膜相当。没有炎症反应或抑制新组织形成。此外，DNA/脂质膜显示出对细菌或念珠菌的抗菌和抗真菌活性。另一方面，无论脂质结构如何，植入大鼠背部后3天，DNA/脂质膜几乎溶解。由于壳聚糖能与DNA结合，因此我们选择壳聚糖代替人工脂质作为与DNA结合的试剂。DNA/壳聚糖复合物对MG-63成骨细胞样细胞没有毒性，仅在大鼠背部引起轻微的组织反应。此外，盐酸柔红霉素复合物插入DNA/壳聚糖复合物。另一方面，还研究了DNA/脂质/聚乳酸膜。这些膜也保持了双螺旋结构，因为盐酸柔红霉素复合物插入DNA/脂质/聚乳酸膜。这些结果表明，DNA/壳聚糖复合物或DNA/脂质/聚乳酸膜可用作DDS材料

项目成果

PBS Buffer Solutions with Different pH Values Can Change Porosity of DNA-chitosan Complexes

不同pH值的PBS缓冲溶液可以改变DNA-壳聚糖复合物的孔隙率

• 发表时间: 2005
• 期刊: Dental Materials Journal 24・3
• 作者: T.Fukushima;T.Hayakawa;M.Kawaguchi;R.Ogura;Y.Inoue;K.Morishita;K.Miyazaki
• 通讯作者: K.Miyazaki

抗菌性を有する床用レジンの試作

具有抗菌性能的地板树脂原型

• 发表时间: 2005
• 期刊: 医学と生物学 149・5
• 作者: 上西秀則;小倉理恵子;豊田美香;長環;井上勇介
• 通讯作者: 井上勇介

Intercalation behavior and tensile strength of DNA-lipid films for the dental application

• DOI: 10.1016/j.biomaterials.2004.01.006
• 发表时间: 2004-11-01
• 期刊: BIOMATERIALS
• 影响因子: 14
• 作者: Fukushima, T;Hayakawa, T;Okahata, Y
• 通讯作者: Okahata, Y

Antifungal activity of DNA-lipid complexes and DNA-lipid films against candida species

• DOI: 10.1002/jbm.a.30394
• 发表时间: 2006-01-01
• 期刊: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
• 影响因子: 4.9
• 作者: Inoue, Y;Fukushima, T;Okahata, Y
• 通讯作者: Okahata, Y