Development of drug delivery system for bone formation using cytokine containing DNA film

点击翻译按钮获取中文摘要

The self-standing, water-insoluble DNA/lipid films containing cytokine were prepared to develop the Drug Delivery System (DDS) for bone formation. The results were as follows : The DNA/lipid films formed intercalation complexes with ethidium bromide. That indicated that the DNA/lipid films maintained a double helical structure. The tensile strength of DNA/lipid films were 0.8-2.4 MPa and were comparable with a commercial available Membrane for guided tissue regeneration in dental use. There were no inflammatory reactions or inhibition of new tissue formation. And also, the DNA/lipid films showed antibacterial and antifungal activities against bacteria or candida species. On the other hand, the DNA/lipid films were almost dissolved three days after implantation in the backs of rats regardless of lipids structure. Since the chitosan can bind easily to DNA, we chose the chitosan as a reagent combined with DNA instead of artificial lipids. The DNA/chitosan complexes were not toxic to MG-63 osteoblast-like cells and caused only a mild tissue response in the backs of rats. And also, the daunorubicin hydrochloride complex intercalated with DNA/chitosan complexes. On the other hand, the DNA/lipid/poly-lactic-acid films were also investigated. These films also maintained a double helical structure because the daunorubicin hydrochloride complex intercalated with DNA/lipid/poly-lactic-acid films. These results suggest the DNA/chitosan complexes or DNA/lipid/poly-lactic-acid films may be useful as DDS materials