Significance of lymphatic invasion on regional lymph node metastasis in cancer of urinary organ by LYVE-limmunohistochemistry

2006年，我们首次通过免疫组化LYVE-1、D2-40、von Willebrand因子和CD 31抗体阐明了肾脏中代谢物的客观分布，这一点长期以来一直不清楚。1.在正常肾脏中，在叶间动脉和弓状动脉周围的间质组织中有丰富的纤维素。它们很少出现在肾皮质的肾小球和肾小管区域。此外，它们在肾间质的结缔组织中几乎不存在。2.在肾癌中，在肿瘤的周围大量发现了肿瘤细胞，然而，它们在肿瘤本身中没有被证明。相反，肿瘤内也显示毛细血管。3.在其他病理状态下也可观察到肾癌细胞浸润，但在终末期肾组织中尤为明显。2007年，我们进一步研究了肾癌细胞淋巴管浸润对区域淋巴结转移的意义。肿瘤周围淋巴管密度增加，淋巴结转移组与淋巴结阴性转移组无差异。比较淋巴结转移组与无淋巴结转移组的肿瘤大小、组织病理学分型、核浸润分级、静脉浸润和淋巴管浸润情况，发现有淋巴结转移组与无淋巴结转移组之间存在一定差异。然而，当我们通过多变量分析进一步分析这种关系时，只有肿瘤周围的淋巴浸润因素被认为是淋巴结转移的原因。我们在进行上述研究的同时，申请了LYVE-1抗体的提高方法的专利。2007年初，它得到了日本政府的批准。

In 2006, we have first elucidated the objective distribution of the lymphatics in the kidney employing immunohistochemistry by LYVE-1, D2-40, von Willebrand Factor and CD 31 antibodies, which has long been obscure. 1. In the normal kidney, the lymphatics are abundant in the interstitial tissues around the interlobar and arcuate arteries. They are seldom present in the glomerular and urinary tubular regions in the renal cortex. In addition, they are almost absent in the interstitium in the renal meddula. 2. In renal carcinoma, the lymphatics are abundantly found at the periphery of the tumor, however, they are not demonstrated within the tumor itself. On the contrary, blood capillary are shown also within the tumor. 3. The lymphatics are demonstrable in the other pathologic conditions, however, among these, its density was extraordinarily noted in the end-stage kidney.In 2007, we further investigated the significance of lymphatic invasion by renal carcinoma cell on regional lymph node metastasis. The lymphatic vessel density increased around the tumor, however, it did not differ between lymph node metastasis group and negative lymph node metastasis group. When we compared the tumor size, histopathologic typing, nuclear atypism grade, venous invasion and lymphatic invasion, some differences were observed between lymph node metastasis group and node negative group. However, when we further analyzed this relationship by multivariate analysis, only the factor of lymphatic invasion around the tumor was considered to be responsible for lymph node metastasis.While we carried out the above study, we applied the patent for the raising methods of LYVE-1 antibody. In the beginning of the year 2007, it was approved by the Japanese government.