权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Screening for modifier genes of polyglutamine-induced neuronal dysfunction using a Drosophila polyglutamine disease model.

使用果蝇多谷氨酰胺疾病模型筛选多谷氨酰胺诱导的神经元功能障碍的修饰基因。

基本信息

批准号：
17590875
负责人：
NAGAI Yoshitaka
金额：
$ 2.24万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

项目摘要

The polyglutamine (polyQ) diseases are a group of inherited neurodegenerative diseases, including Huntington's disease and spinocerebellar ataxias, which are caused by an abnormal expansion of the polyQ stretch in each unrelated disease-causing protein. It has been recently suggested that the neurological phenotypes of polyQ disease patients are caused by reversible neuronal dysfunction rather than neuronal cell death. However, the molecular mechanisms involved in polyQ-induced neuronal dysfunction are not fully understood so far. To investigate the molecular mechanisms of polyQ-induced neuronal dysfunction, we screened for modifier genes of premature death in a Drosophila polyQ disease model, which is a phenotype caused by neuronal dysfunction. We established a GS-polyQ fly line which conditionally expresses an expanded polyQ protein in the nervous system in an RU486-dependent manner using the GeneSwitch system. We then crossed this GS-polyQ fly line with various mutant fly lines which have a partial deletion in their chromosomes (Bloomington deficiency kit,220 lines), and evaluated the survival rate of their offspring. We successfully identified 11 suppressor lines, whose offspring exhibited an improved survival rate compared with the GS-polyQ line. Importantly, nine of these 11 lines did not suppress polyQ-induced neuronal cell death, suggesting that these deleted regions contain genes involved in polyQ-induced neuronal dysfunction but not neuronal cell death. We next screened for genes responsible for the improved survival rate within these 11 deleted chromosomal regions, and selected three candidate genes. We further evaluated the effects of knockdown of these candidate genes using RNAi, and identified a novel gene (Sup5) which is involved in polyQ-induced neuronal dysfunction. We therefore conclude that the Sup5 gene is involved in a novel mechanism of polyQ-induced reversible neuronal dysfunction, providing a novel therapeutic target for the polyQ diseases.

多聚谷氨酰胺（polyQ）疾病是一组遗传性神经退行性疾病，包括亨廷顿氏病和脊髓小脑共济失调，它们是由各不相关致病蛋白的polyQ拉伸异常扩张引起的。最近有研究表明，多q病患者的神经表型是由可逆性神经元功能障碍引起的，而不是神经元细胞死亡。然而，多聚q诱导的神经元功能障碍的分子机制目前还不完全清楚。为了研究polyQ诱导的神经元功能障碍的分子机制，我们在果蝇polyQ疾病模型中筛选了过早死亡的修饰基因，这是一种由神经元功能障碍引起的表型。我们利用GeneSwitch系统建立了一个GS-polyQ蝇系，该蝇系在神经系统中以ru486依赖的方式有条件地表达扩增的polyQ蛋白。然后，我们将该GS-polyQ蝇系与各种染色体部分缺失的突变蝇系（Bloomington缺陷试剂盒，220个系）杂交，并评估其后代的存活率。我们成功地鉴定了11个抑制系，其后代与GS-polyQ系相比表现出更高的存活率。重要的是，这11个品系中有9个没有抑制polyq诱导的神经元细胞死亡，这表明这些缺失的区域包含与polyq诱导的神经元功能障碍有关的基因，而不是神经元细胞死亡。接下来，我们在这11个缺失的染色体区域中筛选与提高存活率有关的基因，并选择了三个候选基因。我们使用RNAi进一步评估了敲除这些候选基因的效果，并鉴定了一个参与多q诱导的神经元功能障碍的新基因（Sup5）。因此，我们得出结论，Sup5基因参与了polyQ诱导的可逆性神经元功能障碍的新机制，为polyQ疾病提供了新的治疗靶点。

项目成果

期刊论文数量（28）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

神経変性疾患のサイエンス

神经退行性疾病科学

DOI：
发表时间：
期刊：
影响因子：
0
作者：
Watanabe;M;Shingo Kikuchi;Wataru Yamori;Kozo Morimoto;Toshiki Yabe;Toshiki Yabe;菊地真吾;Toshiki Yabe;Shingo Kikuchi;Wataru Yamori;H.A.Popiel;I.Mizuta;H.Xiong;T.Chiyonobu;N.Fujikake;S.Kariya;Y.Nagai;I.Mizuta;水澤英洋;葛原茂樹;高橋良輔
通讯作者：
高橋良輔

Multiple candidate gene analysis identifies a-synuclein as a susceptibility gene for sporadic Parkinson's disease.

多个候选基因分析确定α-突触核蛋白是散发性帕金森病的易感基因。

DOI：
发表时间：
2006
期刊：
Hum Mol Genet 15
影响因子：
0
作者：
Mizuta I;Satake W;Nakabayashi Y;Ito C;Suzuki S;Momose Y;Nagai Y;Oka A;Inoko H;Fukae J;Saito Y;Sawabe M;Murayama S;Yamamoto M;Hattori N;Murata M;Toda T.
通讯作者：
Toda T.

Protein transduction domain-mediated delivery of QBP1 suppresses polyglutamine-induced neurodegeneration in vivo