Breeding of a super yeast whichi produced alcohol directly from cellulose

直接从纤维素中生产酒精的超级酵母的选育

• 批准号: 14560076
• 负责人: ARAI Motoo
• 金额: $ 2.3万
• 依托单位: Osaka Prefecture University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14560076/
• 关键词: cellulase; glucosidase; gene; yeast; Aspergillus; セルロース; Aspergillus aculeatus; アルコール

We tried breeding a yeast which produced alcohol directly from cellulose. Cellulase genes were from Aspergillus aculeatus. Expression vector having different nutrition requirement and different expression modes was constructed. Finally, we succced the double expression of 2 endo cellulases. However, the expression of exo cellulases and beta-glucosidase could not be detected. The expression of beta glucosidase was detected slightly when secretion signal was changed. To know the mechanism of expression control of cellulase genes, the mechanism of CREA was investigated.

我们试着培育一种能直接从纤维素中生产酒精的酵母。纤维素酶基因来自棘孢曲霉。构建了具有不同营养需求和不同表达模式的表达载体。最后，我们成功地实现了两种内切纤维素酶的双表达。但未检测到胞外纤维素酶和β-葡萄糖苷酶的表达。当分泌信号发生变化时，β葡萄糖苷酶的表达略有变化。为了解纤维素酶基因表达调控的机理，对CREA的作用机制进行了研究。

项目成果

有用微生物酵素に関する基礎と応用

有用微生物酶的基础知识和应用

• 发表时间: 2004
• 期刊: 日本農芸化学会誌 78
• 作者: 荒井基夫