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Utilization of Microbial Cellodextrin and Cellobiose Phosphorylase

Utilization of Microbial Cellodextrin and Cellobiose Phosphorylase
微生物纤维糊精和纤维二糖磷酸化酶的利用
批准号:
09660101
负责人:
ARAI Motoo
金额:
$1.86万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
1997
资助国家:
日本
项目状态:
已结题
起止时间:
1997 至 1998

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中文摘要
翻译
克隆了纤维二糖磷酸化酶和纤维糊精磷酸化酶基因,并在大肠杆菌中得到了高效表达。这些酶容易从E. coli细胞。利用这些酶,合成了还原末端为对硝基苯酚或14C标记葡萄糖的纤维低聚糖。这些寡糖是阐明纤维素酶水解纤维素机理的有效工具。
英文摘要
The genes of cellobiose phosphorylase and cellodextrin phosphorylase were cloned and these genes expressed effectively in Eschrichia coli. These enzymes were obtained easily from E. coli cells. Using these enzyme, the cellooligosaccharides whose reducing end was p-nitrophenol or 14C-labeled glucose were synthesized. These oligosaccharides was effective tool to elucidate of mechanism of cellulose hydrolysis by cellulases.
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会议论文
T.Kawaguchi et al: "Cloning, Nucleotide Sequence, and Expression of Clostridium thermocellum Cellodextrin Phospherylase"J.Ferment.Bioeng.. 85. 144-149 (1998)
T.Kawaguchi 等:“热纤梭菌纤维糊精磷酸化酶的克隆、核苷酸序列和表达”J.Ferment.Bioeng.. 85. 144-149 (1998)
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T.Kawaguchi et al: "Cloning, Nucleotide Seguence, and Expression of Clostridium thermocellum Cellodextrin Phosphorylase and Its Application of Synthesis…" J.Ferment.Bioeng. 85. 144-149 (1998)
T. Kawaguchi 等人:“热纤梭菌纤维糊精磷酸化酶的克隆、核苷酸序列和表达及其合成应用……”J.Ferment.Bioeng. 85. 144-149 (1998)
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Breeding of a super yeast whichi produced alcohol directly from cellulose
  • 批准号:
    14560076
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 资助金额:
    $2.3万
  • 财政年份:
    2002
  • 负责人:
    ARAI Motoo
  • 依托单位:
Breeding of yeast which ferments directly cellulose
  • 批准号:
    11460049
  • 项目类别:
    Grant-in-Aid for Scientific Research (B).
  • 资助金额:
    $5.89万
  • 财政年份:
    1999
  • 负责人:
    ARAI Motoo
  • 依托单位:
Diversity of Fermented Foods
  • 批准号:
    10041179
  • 项目类别:
    Grant-in-Aid for Scientific Research (A).
  • 资助金额:
    $12.8万
  • 财政年份:
    1998
  • 负责人:
    ARAI Motoo
  • 依托单位:
Biomanufacture of Cotton Fiber by Chemically modified Cellulases
  • 批准号:
    10556022
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 资助金额:
    $6.21万
  • 财政年份:
    1998
  • 负责人:
    ARAI Motoo
  • 依托单位:
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