Utilization of Microbial Cellodextrin and Cellobiose Phosphorylase

微生物纤维糊精和纤维二糖磷酸化酶的利用

• 批准号: 09660101
• 负责人: ARAI Motoo
• 金额: $ 1.86万
• 依托单位: Osaka Prefecture University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09660101/
• 关键词: Phosphorylase; Cellobiose; Cellodextrin; Clostridium

The genes of cellobiose phosphorylase and cellodextrin phosphorylase were cloned and these genes expressed effectively in Eschrichia coli. These enzymes were obtained easily from E. coli cells. Using these enzyme, the cellooligosaccharides whose reducing end was p-nitrophenol or 14C-labeled glucose were synthesized. These oligosaccharides was effective tool to elucidate of mechanism of cellulose hydrolysis by cellulases.

克隆了纤维素二糖磷酸化酶和纤维素糊精磷酸化酶基因，并在大肠杆菌中有效表达。这些酶很容易从大肠杆菌细胞中获得。利用该酶合成了还原端为对硝基酚或14c标记葡萄糖的纤维低聚糖。这些低聚糖是阐明纤维素酶水解纤维素机理的有效工具。

项目成果

T.Kawaguchi et al: "Cloning, Nucleotide Sequence, and Expression of Clostridium thermocellum Cellodextrin Phospherylase"J.Ferment.Bioeng.. 85. 144-149 (1998)

T.Kawaguchi 等：“热纤梭菌纤维糊精磷酸化酶的克隆、核苷酸序列和表达”J.Ferment.Bioeng.. 85. 144-149 (1998)

T.Kawaguchi et al: "Cloning, Nucleotide Seguence, and Expression of Clostridium thermocellum Cellodextrin Phosphorylase and Its Application of Synthesis…" J.Ferment.Bioeng. 85. 144-149 (1998)

T. Kawaguchi 等人：“热纤梭菌纤维糊精磷酸化酶的克隆、核苷酸序列和表达及其合成应用……”J.Ferment.Bioeng. 85. 144-149 (1998)

T. Kawaguchi et al: "Cloning and Nucleotide Sequence, and Expression of Clostridium thermocellum Phosphorylase"J. Ferment, Bioeng.. 85. 144-149 (1998)

T. Kawaguchi 等人：“热纤梭菌磷酸化酶的克隆和核苷酸序列以及表达”J。