Studies on Cellulase Inhibitors

纤维素酶抑制剂的研究

• 批准号: 06660119
• 负责人: ARAI Motoo
• 金额: $ 1.34万
• 依托单位: University of Osaka Prefecture
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06660119/
• 关键词: Cellulase; Inhibitor; インヒビター

Cellulase consits of many components which have different action pattern. However, mechanism of cellulose hydrolysis by cellulases is still not clear. So, we tried to synthesise cellulase inhibitors from deoxynojirimysin (SGI) and cellooligosaccharides using transglucosylation of cellulase.1. Cellulase inhibitors were synthesized from 2% SGI,10% Cellooligosaccharides, 2.5% cellulase, 30% DMSO in acetate buffer. Inhibitors were purified by ion exchage column chromatography, gel filtration and HPLC.We obtained 2 novel inhibiotrs.2. Structure of inhibitors were estimated as 4-0-beta-cellotriosyl-SGI and 4-0-beta-cellotetraosyl-SGI by composition analysis, beta-glucosidase hydrolysis and methylation analysis.3. New effective synthesis of inhibitors using cellodextrinphosphorylase were estimated. Inhibitors were G_nSGI.4. CM-cellase from Aspergillus aculeatus were potently inhibited by G_3SGI and G_4SGI.

纤维素酶由许多具有不同作用模式的组分组成。然而，纤维素酶降解纤维素酶的作用机理尚不清楚。因此，我们试图通过纤维素酶的葡萄糖转移反应，从脱氧野生肌素(SGI)和纤维低聚糖中合成纤维素酶抑制剂。以2%SGI、10%低聚纤维素糖、2.5%纤维素酶、30%DMSO为原料，在醋酸盐缓冲溶液中合成纤维素酶抑制剂。通过离子交换柱层析、凝胶过滤和高效液相色谱分离纯化，得到2个新的抑制剂。通过组成分析、β-葡萄糖苷酶水解和甲基化分析，确定其结构为4-0-β-纤维三糖-SGI和4-0-β-纤维四糖-SGI。利用纤维糊精磷酸化酶对新的有效合成抑制剂进行了评估。抑制剂为G_nSGI.4。G_3SGI和G_4SGI对尖锐曲霉CM-纤维素酶有较强的抑制作用。

项目成果

T.Kawaguchi: "Structures and Activity of Cellulase Inhibitors Enzymatically Synthesized from Celloaligosaccharioles and 1-Deoxynojrimycin" Biosci.Biotech.Biochem.60. 344-346 (1996)

T.Kawaguchi：“由纤维寡糖和 1-脱氧野吉霉素酶促合成的纤维素酶抑制剂的结构和活性”Biosci.Biotech.Biochem.60。

T,Kawaguchi et.al.: "Structures and Activity of cellulose Inhibiters Engymatically Synthesized from cellocligosacchrides and 1-Deoxynojirimycin" Biosci.Biotech.Biochem.60. 344-346 (1996)

T,Kawaguchi 等人：“由纤维寡糖和 1-脱氧野尻霉素酶促合成的纤维素抑制剂的结构和活性”Biosci.Biotech.Biochem.60。

T.Kawaguchi et al: "Structure and Activity of Cellulase Inhibitors Enzymatically Synthesized from Cellooligosaccharides and deoxynojirimycin" Biosci.Biotech.Biochem. 60. 344-346 (1996)

T.Kawaguchi 等人：“由纤维寡糖和脱氧野尻霉素酶促合成的纤维素酶抑制剂的结构和活性”Biosci.Biotech.Biochem。