Studies on Cellulase Inhibitors

纤维素酶是由多种组分组成的，它们具有不同的作用方式。然而，纤维素酶水解纤维素的机理尚不清楚。因此，我们尝试利用纤维素酶的转葡萄糖基化作用，以脱氧野尻霉素（SGI）和纤维寡糖为原料合成纤维素酶抑制剂.以2%SGI、10%纤维低聚糖、2.5%纤维素酶、30%DMSO为原料，在醋酸缓冲液中合成了纤维素酶抑制剂。通过离子交换柱层析、凝胶过滤和高效液相色谱对抑制剂进行了分离纯化，得到了2个新的抑制剂.通过组成分析、β-葡萄糖苷酶水解和甲基化分析，确定抑制剂的结构为4-0-beta-cellotriosyl-SGI和4-0-beta-cellotetraosyl-SGI.估计了利用纤维糊精磷酸化酶合成抑制剂的新的有效途径。抑制剂是G_nSGI.4。G_3SGI和G_4SGI对棘孢曲霉CM-纤维素酶有较强的抑制作用。

Cellulase consits of many components which have different action pattern. However, mechanism of cellulose hydrolysis by cellulases is still not clear. So, we tried to synthesise cellulase inhibitors from deoxynojirimysin (SGI) and cellooligosaccharides using transglucosylation of cellulase.1. Cellulase inhibitors were synthesized from 2% SGI,10% Cellooligosaccharides, 2.5% cellulase, 30% DMSO in acetate buffer. Inhibitors were purified by ion exchage column chromatography, gel filtration and HPLC.We obtained 2 novel inhibiotrs.2. Structure of inhibitors were estimated as 4-0-beta-cellotriosyl-SGI and 4-0-beta-cellotetraosyl-SGI by composition analysis, beta-glucosidase hydrolysis and methylation analysis.3. New effective synthesis of inhibitors using cellodextrinphosphorylase were estimated. Inhibitors were G_nSGI.4. CM-cellase from Aspergillus aculeatus were potently inhibited by G_3SGI and G_4SGI.