Breeding of yeast which ferments directly cellulose

直接发酵纤维素的酵母的选育

• 批准号: 11460049
• 负责人: ARAI Motoo
• 金额: $ 5.89万
• 依托单位: Osaka Prefecture University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11460049/
• 关键词: cellulose; cellulase; gene; cloning; expression; セルロース; セルラーゼ; キシラナーゼ; マンノシダーゼ; 遺伝子; 糸状菌; バイオマス

For utilization of biomass cellulosic substances are important because they are dominant organic substances on the earth. Cellulases consist of complex system and they hydrolyze cellulosic substances synergistically. We isolated Aspergillus aculeatus which shows synergism with Trichoderma cellulase. When the cellulase from Aspergillus aculeatus was mixed with Trichoderma cellulase, enzyme showed potent synergisty and hydrolyzed completely cellulosic substances. The research elucidated essential cellulase components. The enzyme genes were cloned and then these genes were introduced into yeast cells. Finally, we can expected the yeast which produced ethanol directly from cellulose.The genes of 1 β-glucosidase, 2 Avicelases, 2 CM-cellulases, β-mannosidase, 1 xylanase were cloned. These genes were expressed in yeast cells. β-mannosidase gene was introduced into Aspergillus. The enzyme production improved in fungal cells.

对于生物质的利用，纤维素物质是重要的，因为它们是地球上占主导地位的有机物质。纤维素酶是一个复杂的酶系，它们协同水解纤维素类物质。我们分离到了与木霉纤维素酶具有协同作用的棘孢曲霉。将棘孢曲霉纤维素酶与木霉纤维素酶混合，酶具有很强的协同作用，能完全水解纤维素类物质。本研究阐明了纤维素酶的基本组分。克隆酶基因，然后将这些基因导入酵母细胞。克隆了1个β-葡萄糖苷酶基因、2个Avicelase基因、2个CM-纤维素酶基因、1个β-甘露糖苷酶基因、1个木聚糖酶基因。这些基因在酵母细胞中表达。将β-甘露糖苷酶基因导入曲霉菌。真菌细胞中的酶产量提高。

项目成果

G.Takada et al: "Cloning and Sequencing of β-Mannosidase Gene from Asp. aculeatus No.F.50"Biosci.Biotechnol.Biochem.. 63. 206-209 (1999)

G. Takada 等：“来自棘孢曲霉 No.F.50 的 β-甘露糖苷酶基因的克隆和测序”Biosci.Biotechnol.Biochem.. 63. 206-209 (1999)

T. Ooi, et. al.: "Analysis of 5'-upstream non-coding region of FI-carboxy methyl cellulase from Aspergillus aculeatus"Biotechnol. Lett.. 21. 735-739 (1999)

T. Ooi 等。

G.Takada et al.: "Cloning and Sequencing of β-Mannosidase Gene from Asp.aculeatus No.F-50"Biosci.Biotechnol.Biochem.. 63. 206-209 (1999)

G. Takada 等：“来自棘孢曲霉 No.F-50 的 β-甘露糖苷酶基因的克隆和测序” Biosci.Biotechnol.Biochem.. 63. 206-209 (1999)

A.Ohnishi et al.: "Analysis of a Catalytic Acidic Pair in the Active Center of Cellulase from Asp.aculeatus"Biosci.Biotechnol.Biochem.. 63. 2157-2162

A.Ohnishi 等人：“来自 Asp.aculeatus 的纤维素酶活性中心的催化酸性对的分析”Biosci.Biotechnol.Biochem.. 63. 2157-2162

A.Ohnishi et al: "Analysis of a Catalytic Acidic Pair in the Active Center of Cellulase from Asp.aculeatas"Biosci.Biotechnol.Biochem.. 63. 2157-2162 (1999)

A.Ohnishi 等人：“来自 Asp.aculeatas 的纤维素酶活性中心的催化酸性对的分析”Biosci.Biotechnol.Biochem.. 63. 2157-2162 (1999)