Studies on the structure and functions of cellulase

结果如下：(1)克隆了针叶鱼乙酰化酶基因，并对其核苷酸序列进行了分析。该基因在酵母中表达。(2)黑曲霉β -葡萄糖苷酶亚位点严格识别葡萄糖，这可能反映了底物特异性。(3)克隆了a.o oryzae的两个内切葡聚糖酶基因，并对其核苷酸序列进行了分析。(4)克隆了热胞梭菌纤维素酶基因（CelE），并对其核苷酸序列进行了分析。该基因在大肠杆菌中表达。(5)克隆了T.reesei内切葡聚糖酶基因，并对其核苷酸序列进行了分析。表达了该基因。(6)真菌中3种内切葡聚糖酶的底物特异性。酶解机制取决于底物的结构。(7)寻找烟草培养细胞固定期高表达的cDNA，并分离得到该DNA。(8)纤维素低氢寡糖因其无水标记作用，可用于阐明纤维素酶的反应机理。(9)荚膜红杆菌能有效地从纤维二糖中产生氢气。（10）从黑曲霉中纯化了3种纤维素酶，并对其特性进行了分析。（11）以gilvus （CG）和农杆菌（AT）的葡萄糖苷酶为原料制备了三种嵌合酶。研究了它们的性质。(HAYASHI)

The results were as follows :(1) Avicelase gene of A.aculeatus was cloned and elucidated its nucleotide sequence. The gene was expressed in yeast. (ARAI)(2) Subsite of beta-glucosidase of A.niger recognized glucose strictly and this may reflect substrate specificity. (OKADA)(3) Two endoglucanase genes of A.oryzae were cloned and its nucleotide sequences were elucidated.(4) Cellulase gene (CelE) of Clostridium thermocellum was cloned and elucidated its nucleotide sequence. The gene was expressed in Escherichia coli. (OHMIYA)(5) Endoglucanase gene of T.reesei was cloned and elucidated its nucleotide sequence. The gene was expressed. (MORIKAWA)(6) Substrate specificities of 3 endoglucanases from fungi. Enzymatic hydrolysis mechanism depended on the structure of substrate. (KANDA)(7) Highly expressed cDNA at stationary phase of tabacco culture cell was searched and that DNA was isolated. (SHINMYO)(8) Celloanhydrooligosaccharides were useful for elucidation of reaction mechanism of cellulases because of marking effect of anhydroring. (ADUMA)(9) Rhodobacter capsulatus produced effectively hydrogen gas from cellobiose. (OHSHIMA)(10) Three cellulases from A.niger were purified and there characteristics were elucidated. (OGAWA)(11) Three type of chimeric enzymes were prepared from glucosidases from Celluvibrio gilvus (CG) and Agrobacterium tumefaciens (AT). There properties were studied. (HAYASHI)