Study on simultaneous utilization of a mixed sugar in canned syrup by sucrose-high-consumable lactic acid bacteria

蔗糖-高耗乳酸菌同时利用罐装糖浆中混合糖的研究

• 批准号: 14560284
• 负责人: UENO Takashi
• 金额: $ 1.92万
• 依托单位: Hakodate National College of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14560284/
• 关键词: sucrose; lactic acid; syrup; a mixed sugar; Lactobacillus paracasei; Lactococcus lactis; catabolite repression

1. Effect of carbon source on production of sucrose-hydrolyzable enzymeLactobacillus paracasei 1532 and Lactococcus lactis IFO12007 were cultured with 10 g l^<-1> sucrose, glucose, cellobiose or Methyl β-D-Glucopyranoside as sole carbon source, and enzyme activities were measured. Lb.paracasei 1532 showed high activity of sucrose-hydrolyzable enzyme when sucrose or cellobiose was used as sole carbon source. The strain also produced the enzyme when glucose was used. Therefore the enzyme of the strain is probably constitutive enzyme. Since the strain was capable to grow with Methyl β-D-Glucopyranoside, a part of the enzyme could be β-glucosidase. Lc.lactis IFO12007 grew very slowly when sucrose was used as sole carbon source, and the enzyme activity was very low.2. Kinetic analysis in continuous production of lactic acid from canned pineapple syrupLb.paracasei 1532 was cultivated with 20 g l^<-1> sugar using canned pineapple syrup after precultivation with glucose. Then continuous cultivation was carried out feeding 100 g l^<-1> sugar of concentrated medium. All carbon sources including sucrose were metabolized until washout at 0.20 h^<-1> of dilution rate. As a result of kinetic analysis specific growth rate (μ_m) and Michaelis constant (K_s) were 0.24 h^<-1> and 2.4 g l^<-1>, respectively. Continuous cultivation by Lc.lactis IFO12007 was carried out in the same way after precultivation with sucrose. While glucose and fructose were completely consumed, sucrose could not be metabolized and accumulated in the culture broth. μ_m and _Ks were -0.04 h^<-1> and -53.06 g l^<-1>, respectively. Kinetic analysis also indicated that catabolite repression of sucrose by glucose and fructose occurred in Lc.lactis IFO12007 but no catabolite repression was observed in Lb.paracasei 1532.

1.碳源对副干酪乳杆菌（Lactobacillus paracasei）1532和乳酸乳球菌（Lactococcus lactis）IFO 12007产蔗糖水解酶的影响以<-1>蔗糖、葡萄糖、纤维二糖和甲基β-D-吡喃葡萄糖苷为唯一碳源，测定酶活。副干酪乳杆菌1532在以蔗糖或纤维二糖为唯一碳源时表现出较高的蔗糖水解酶活性。当使用葡萄糖时，该菌株也产生酶。因此，该菌株的酶可能是组成型酶。由于该菌株能够与甲基β-D-吡喃葡萄糖苷一起生长，因此部分酶可能是β-葡萄糖苷酶。以蔗糖为唯一碳源时，乳酸乳杆菌IFO 12007生长缓慢，酶活性很低.从罐装菠萝糖浆中连续生产乳酸的动力学分析在<-1>用葡萄糖预培养后，使用罐装菠萝糖浆用20 g l/L糖培养副干酪乳杆菌1532。然后进行连续培养，进料100 g/L<-1>糖的浓缩培养基。包括蔗糖在内的所有碳源在0.20 h-1稀释速率下代谢直至洗脱<-1>。动力学分析结果表明，比生长速率（μ m）和米氏常数（K_s）分别为0.24h ~（-1）<-1>和2.4g ~（-1）<-1>。在用蔗糖预培养后，以相同的方式进行乳酸乳杆菌IFO 12007的连续培养。当葡萄糖和果糖被完全消耗时，蔗糖不能被代谢并在培养液中积累。μ_m和_Ks分别为-0.04 h-1<-1>和-53.06 g-1<-1>。动力学分析还表明，在乳酸乳杆菌IFO 12007中发生葡萄糖和果糖对蔗糖的分解代谢物阻遏，但在副干酪乳杆菌1532中未观察到分解代谢物阻遏。