Search for genes associated with genome copy number imbalance in human cancer

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Many of human carcinomas show DNA aneuploidy with DNA index 1.3 or more. We think that this is one of basic feature of human carcinoma, however, little is known about the mechanism of acquisition of DNA aneuploidy. For detecting overall genome copy number aberration in detail and approaching to genes associated with the mechanism of acquisition of DNA aneuploidy, some carcinomas have been analyzed by comparative genomic hybridizations. The head investigator and other researchers (we) found and reported that a recurrent DNA copy number aberration (gain) at 18p in mucinous type colorectal carcinoma with DNA aneuploidy. Furthermore, we have reported that centrosome hyperamplification associated with DNA ploidy, 20q13, on which the Aurora-A gene is located, copy number increasing or tumor recurrence in bladder cancer. In addition to them, the head investigator took chances for publications about DNA aneuploidy in solid tumors and technical notes for DNA measurement with cytometer in Japanese. We have tried other experiments, for example, an experiment to induce DNA ploidy abnormality using cultured cell or to detect genome copy aberrations in precancerous lesions. We are preparing for publishing based on results from these experiments.