Search for genes associated with genome copy number imbalance in human cancer

许多人类肿瘤显示DNA非整倍体，DNA指数为1.3或更高。我们认为这是人类肿瘤的基本特征之一，但对DNA非整倍体获得的机制还知之甚少。为了详细检测整个基因组拷贝数异常并探讨与DNA非整倍体获得机制相关的基因，人们通过比较基因组杂交分析了一些癌症。本课题组组长和其他研究人员发现并报道了粘液型结直肠癌中18 p处的DNA拷贝数异常（增加），并伴有DNA非整倍体。此外，我们还报道了中心体过度扩增与膀胱癌中Aurora-A基因所在的20 q13 DNA倍体、拷贝数增加或肿瘤复发相关。除此之外，首席研究员还利用机会发表了关于实体瘤中DNA非整倍体的出版物和使用细胞仪进行DNA测量的技术说明（日语）。我们还尝试了其他实验，例如，使用培养细胞诱导DNA倍性异常或检测癌前病变中的基因组拷贝畸变的实验。我们正在准备根据这些实验的结果发表。

Many of human carcinomas show DNA aneuploidy with DNA index 1.3 or more. We think that this is one of basic feature of human carcinoma, however, little is known about the mechanism of acquisition of DNA aneuploidy. For detecting overall genome copy number aberration in detail and approaching to genes associated with the mechanism of acquisition of DNA aneuploidy, some carcinomas have been analyzed by comparative genomic hybridizations. The head investigator and other researchers (we) found and reported that a recurrent DNA copy number aberration (gain) at 18p in mucinous type colorectal carcinoma with DNA aneuploidy. Furthermore, we have reported that centrosome hyperamplification associated with DNA ploidy, 20q13, on which the Aurora-A gene is located, copy number increasing or tumor recurrence in bladder cancer. In addition to them, the head investigator took chances for publications about DNA aneuploidy in solid tumors and technical notes for DNA measurement with cytometer in Japanese. We have tried other experiments, for example, an experiment to induce DNA ploidy abnormality using cultured cell or to detect genome copy aberrations in precancerous lesions. We are preparing for publishing based on results from these experiments.