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Immunohistochemical syudy on differential diagnosis of traumatic or intrinsic cerebral hematoma

外伤性或内源性脑血肿鉴别诊断的免疫组化研究

基本信息

批准号：
14570390
负责人：
OGATA Mamoru
金额：
$ 1.86万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14570390/
关键词：
Traumatic cerebral hematoma Intrinsic cerebral hemorrhage Angiotensin convertingenzyme Amyloid precursor protein Neuron-specific enolase Heme oxigenase-1 Exitatory amino acid transporter 2 Glial fibrillary acidic protein C9

项目摘要

1.Iminunohistochemical studies on heine oxigenase-l(HO-1)Immunostaining for H0-1, one of the heat shock protein family, labelled neurocytes and astrocytes in cases of head injury, whereas it did not label those cells in control cases.Therefore, it is suggested that imniunostaining for HO-1 should be useful for diagnosing head injury.2.Immunohistochemical studies on angiotensin converting enzyme(ACE) : Immunostaining for ACE, which has been reported to label unstable plaques of arteriosclerosis, was tested using sections from coronary arteries in cases of ischemic heart diseases.In all cases of acute myocardial infarction, staining for ACE labelled cells in plaques of arteriosclerosis.Therefore, it is suggested that immunostaining for ACE should be useful for detection of unstable plaques in cerebral arteries in cases of intrinsic cerebral hemorrhage.3.Differential diagnosis of intrinsic or traumatic cerebral heniatoma Sections from cerebrum in 5 traumatic and 5 intrinsic cases of cerebral hematoma.Necrosis of cerebral arteries were detected in 2 intrinsic cases by Victria Blue staining, whereas did not in traumatic cases.Immunostaining for amyloid precursor protein(APP)and neuron-specific enolase(NSE)labelled varicose axons and axonal bulb in 3 traumatic cases.However, it also labelled axonal bulb in 1 intrinsic case with cerebral herniation.Immunostaining for ACE labelled cells in plaques of arteriosclerosis in 2 intrinsic cases.However, it also labelled cells in 1 intrinsic case with cerebral hematoma of an old woman.Immunostaining for exitatory amino acid transporter 2(EAAT2)and glial fibrillary acidic protein(GFAP)labelled various astorcytes, although no specific changes to intrinsic or traumatic cases were observed.It is suggested that both examinations of pathological changes of cerebral arteries and examinations of axonal changes due to head injury should be necessary for differential dignosis of intrinsic or traumatic cerebral hematoma.

1.海涅氧合酶-1（HO-1）免疫组化研究：H 0 -1免疫组化标记脑损伤后神经胶质细胞和星形胶质细胞，而对照组未见标记，提示HO-1免疫组化对脑损伤的诊断有一定的价值。ACE的免疫染色已被报道标记动脉硬化的不稳定斑块，使用缺血性心脏病病例的冠状动脉切片进行了测试。在所有急性心肌梗死病例中，动脉硬化斑块中的ACE标记细胞染色。因此，提示ACE免疫组化对脑内血肿患者脑动脉内不稳定斑块的检测有一定的价值。3.脑内血肿与外伤性血肿的鉴别诊断Victria Blue染色显示2例内源性脑出血患者脑动脉坏死，3例外伤性脑出血患者脑动脉硬化，5例外伤1例脑内型脑疝患者的轴索球也被标记，2例脑内型患者的动脉硬化斑块中也有ACE标记细胞，用兴奋性氨基酸转运蛋白2（EAAT 2）和胶质细胞酸性蛋白（GFAP）免疫组化标记星形胶质细胞，对外伤性或内源性病例无特异性改变，提示脑损伤时应同时检查脑动脉病变和轴索改变，对鉴别诊断脑内血肿与外伤性脑血肿有重要意义。