Immunohistochemical syudy on differential diagnosis of traumatic or intrinsic cerebral hematoma

1.热休克蛋白家族H0-1标记神经细胞和星形胶质细胞的免疫组织化学研究。在脑损伤患者中，H0-1标记神经细胞和星形胶质细胞。因此，HO-1免疫组织化学染色对脑损伤的诊断有帮助。2.血管紧张素转换酶的免疫组织化学研究：血管紧张素转换酶的免疫组织化学研究：血管紧张素转换酶的免疫组织化学研究：血管紧张素转换酶是一种标记动脉硬化不稳定斑块的免疫组织化学。在所有急性心肌梗死患者的冠状动脉切片上，血管紧张素转换酶标记的细胞都在动脉粥样硬化的斑块中。因此，在所有的急性心肌梗死病例中，血管紧张素转换酶标记的细胞都在动脉粥样硬化的斑块中。因此，在所有的急性心肌梗死患者中，血管紧张素转换酶的免疫组织化学研究3.5例外伤性脑血管瘤和5例内源性脑血肿的脑组织切片的鉴别诊断。2例内源性脑动脉坏死，2例内源性脑动脉未见坏死。3例内源性脑出血，用淀粉样前体蛋白(APP)和神经元特异烯醇化酶(NSE)标记轴突和轴突。1例内源性脑出血，用免疫组织化学方法标记轴突。2例内源性脑出血患者的动脉硬化斑块中，用免疫组织化学方法标记血管紧张素转运蛋白(ACE)标记细胞。2例内源性脑出血患者的动脉硬化斑块内有ACE标记细胞EAAT2和GFAP免疫组织化学标记各种星形细胞，但对脑内血肿和外伤性脑血肿未见特异性改变。提示脑内血肿和外伤性脑血肿的鉴别诊断应结合脑动脉病理改变和脑外伤后轴索改变。

1.Iminunohistochemical studies on heine oxigenase-l(HO-1)Immunostaining for H0-1, one of the heat shock protein family, labelled neurocytes and astrocytes in cases of head injury, whereas it did not label those cells in control cases.Therefore, it is suggested that imniunostaining for HO-1 should be useful for diagnosing head injury.2.Immunohistochemical studies on angiotensin converting enzyme(ACE) : Immunostaining for ACE, which has been reported to label unstable plaques of arteriosclerosis, was tested using sections from coronary arteries in cases of ischemic heart diseases.In all cases of acute myocardial infarction, staining for ACE labelled cells in plaques of arteriosclerosis.Therefore, it is suggested that immunostaining for ACE should be useful for detection of unstable plaques in cerebral arteries in cases of intrinsic cerebral hemorrhage.3.Differential diagnosis of intrinsic or traumatic cerebral heniatoma Sections from cerebrum in 5 traumatic and 5 intrinsic cases of cerebral hematoma.Necrosis of cerebral arteries were detected in 2 intrinsic cases by Victria Blue staining, whereas did not in traumatic cases.Immunostaining for amyloid precursor protein(APP)and neuron-specific enolase(NSE)labelled varicose axons and axonal bulb in 3 traumatic cases.However, it also labelled axonal bulb in 1 intrinsic case with cerebral herniation.Immunostaining for ACE labelled cells in plaques of arteriosclerosis in 2 intrinsic cases.However, it also labelled cells in 1 intrinsic case with cerebral hematoma of an old woman.Immunostaining for exitatory amino acid transporter 2(EAAT2)and glial fibrillary acidic protein(GFAP)labelled various astorcytes, although no specific changes to intrinsic or traumatic cases were observed.It is suggested that both examinations of pathological changes of cerebral arteries and examinations of axonal changes due to head injury should be necessary for differential dignosis of intrinsic or traumatic cerebral hematoma.