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Studies on morphological changes of the nerve fibers (axon and myelin) after head injury.

头部损伤后神经纤维（轴突和髓磷脂）形态变化的研究。

基本信息

批准号：
06670463
负责人：
OGATA Mamoru
金额：
$ 1.41万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670463/
关键词：
Brain injury Nerve fiber Axon Myelin Forsic pathology Immunohistochemistry Diffuse axonal injury 鍍銀染色

项目摘要

1. Morphological changes of the nerve fibers after head injury : The blocks of the corpus callosum which had been taken in autopsy cases were stained for axon with Holmes' silver method and counter-stained for myelin with luxol fast blue (LFB). Axonal retraction balls (RB) and/or myelin globoids (MG) were detected in sections of patients who survived for more than 2 days after head injury. In addition, all of three axonal changes, i.e.swelling, coarse varicosities and waving, were observed in sections of those who survived for more than 5 hours. These findings were not apparent in sections of control patients.2. Immunohistochemical studies with antibodies to axonal components on damage after head injury : To detect early posttraumatic axonal changes, antibodies to several axonal components such as neuron-specific enolase (NSE), subunits of neurofilament (NF), mitochondria and Alzheimer precursor protein (APP) were employed. Preliminary examination showed that NSE,NF-M and APP could be useful markers for detecting early axonal changes, and that NSE staining found to be most sensitive. Further examination using NSE staining revealed that the antibody to NSE could label RB and swollen axons in sections of patients who survived for only 1 hour after head trauma.3. Immunohistochemical studies with antibodies to myelin components on damage after head injury : To detect posttraumatic myelin changes, antibodies to myelin basic protein and proteolipid protein were employed. MG was identified by both of the myelin immunostaining as well as LFB in sections of patients who survived for more than 2 days. These myelin markers, together with axon markers such as NSE,should be quite useful for estimating the duration of posttraumatic survival.

1. 脑损伤后神经纤维形态学变化：解剖病例取胼胝体块，用Holmes' s银法对轴突进行染色，用luxol耐晒蓝（LFB）对髓磷脂进行反染色。在脑损伤后存活2天以上的患者的切片中检测到轴突回缩球（RB）和/或髓磷脂球（MG）。此外，存活超过5小时的小鼠，在切片上观察到肿胀、粗大的静脉曲张和波浪状三种轴突变化。这些发现在部分对照患者中不明显。脑损伤后轴突成分抗体的免疫组化研究：为了检测创伤后早期轴突的变化，采用了针对神经元特异性烯醇化酶（NSE）、神经丝亚基（NF）、线粒体和阿尔茨海默前体蛋白（APP）等轴突成分的抗体。初步检查显示，NSE、NF-M和APP可作为检测早期轴突变化的有用标志物，其中以NSE染色最为敏感。进一步用NSE染色检查发现，在头部创伤后仅存活1小时的患者的切片中，NSE抗体可以标记RB和肿胀的轴突。脑损伤后髓磷脂成分抗体的免疫组化研究：为了检测创伤后髓磷脂的变化，使用了髓磷脂基础蛋白和蛋白脂蛋白抗体。在存活2天以上的患者切片中，髓磷脂免疫染色和LFB均可识别MG。这些髓磷脂标记物，连同轴突标记物如NSE，对于估计创伤后生存时间应该是非常有用的。