Immunohistochemical studies on changes of the components of the nervous system after head injury and their application to estimating the duration of posttraumatic survival.

头部损伤后神经系统组成变化的免疫组织化学研究及其在估计创伤后生存持续时间中的应用。

• 批准号: 08670494
• 负责人: OGATA Mamoru
• 金额: $ 1.47万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670494/
• 关键词: Head in jury; Difuse axonal in jury; Axon; Myelin; Astrocyte; Immunohistochemistry; Neuron-specific enolase; Glial fibrillary acidic protein; S100蛋白; ビメンチン; ラミニン; ニューロフィラメント; アミロイド・プレカーサー蛋白

1.Immunohistochemical studies on changes of axons after brain injury : Eight antibodies against components of axons were tested to determine whether the antibodies can detect early posttraumatic axonal changes. Preliminary examination showed that immunostaining for neuron-specific enolase (NSE), M subunit of neurfilament (NF) and Alzheimer precursor protein (APP) could be useful markers for detection of early axonal changes, and that NSE staining found to be most sensitive. Further examination revealed that NSE staining can label so-called axomal bulbs and swollen axons in cases with only 0.5 hour's survival after head trauma.2.Immunohistochemical studies on changes of mylins after head injury : Antibodies against mylin basic protein and proteolipid protein were employed. Myelin globoids were identified by both of the immunostaining for myelin as well as staining with Luxol Fast Blue in cases with more than 2 days' survival after brain injury.3.Immunohistochemical studies on changes of astrocytes after brain injury : Antibodies against glial fibrillary acidic protein (GFAP), S100 protein, vimentin and laminin were used. Immunostaining for GFAP and S100 labelled both reactive astrocytes in cases of head injury with more than 7 days' survival and swollen astrocytes in cases with 9 hours' to 7 days' survival. Further, in cases with less than 1 hour's survival, clasmatodendrosis and pyknosis of the nucleus were detected by GFAP and S100 staining. Immunostaining for vimentin and laminin also labelled these astrocytes. These results suggest that immunostaining for astrocytes should be quite useful for evaluating brain damage after head injury and for estimating the duration of posttraumatic survival.

1.脑损伤后轴突变化的免疫组化研究：检测8种针对轴突成分的抗体，以确定抗体是否能检测早期创伤后轴突变化。初步检查显示，神经元特异性烯醇化酶（NSE）、神经丝M亚基（NF）和阿尔茨海默病前体蛋白（APP）的免疫染色可作为检测早期轴突变化的有效标志物，其中NSE染色最敏感。进一步检查发现，在头部创伤后仅存活0.5小时的病例中，NSE染色可以标记出所谓的轴突球和肿胀的轴突。脑损伤后mylins变化的免疫组化研究：采用针对mylin碱性蛋白和蛋白脂质蛋白的抗体。脑损伤后存活2 d以上的患者髓磷脂免疫染色及Luxol快速蓝染色均可鉴定髓磷脂球蛋白。脑损伤后星形胶质细胞变化的免疫组化研究：使用抗胶质纤维酸性蛋白（GFAP）、S100蛋白、vimentin和层粘连蛋白抗体。GFAP和S100免疫染色在存活超过7天的头部损伤中标记出反应性星形胶质细胞，在存活9小时至7天的头部损伤中标记出肿胀的星形胶质细胞。此外，在存活时间小于1小时的病例中，通过GFAP和S100染色检测到核枝轴缩和核固缩。免疫染色的波形蛋白和层粘连蛋白也标记这些星形胶质细胞。这些结果表明星形胶质细胞的免疫染色对于评估脑损伤后的脑损伤和估计创伤后生存时间是非常有用的。