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Determination of cause of death and estimation of survival period by the use of immunohistochemical markers of glial cells

利用神经胶质细胞的免疫组织化学标记物确定死亡原因并估计生存期

基本信息

批准号：
12670398
负责人：
OGATA Mamoru
金额：
$ 2.18万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

项目摘要

1. Changes of astrocytes (AC) in cases of short survival period: Changes of AC, especially pyknosis of the nucleus, in cases of death due to several cases were investigated. The formalin-fixed brains from 48 cases of death due to hanging, strangulation, drowning, ex-sanguination and so on were examined. Paraffin-embedded sections from the corpus callosum were immunostained by the use of an antibody against glial fibrillary acidic protein (GFAP). Pyknosis of the nucleus of AC was detected in 27 cases. 25 out of these 27 cases were those of less than 1 hour of survival. Among them, nuclear pyknosis were found in all cases of death due to strangulation. Further, in cases of death due to exsanguination, pyknosis was detected in all cases with less than 1 hour of survival, whereas in no cases with more than 1 hour of survival. Therefore, it is suggested that detection of nuclear pyknosis of AC should be useful for estimation of survival period.2. Detection of nuclear pyknosis of AC in several sections: In a case of a few minutes of survi val with death due to exsanguination, pyknosis of the nucleus of AC was found in sections from the corpus callosum and cerebral white matter, whereas not in those from brain stem nor cere bellum. On the other hand, in a case of instantaneous death due to gunshot, pyknosis of the nucleus of AC was found in all sections from the corpus callosum, cerebral white matter, brain stem and cerebel lum.3. Investigation of changes of glial cells: Nuclear pyknosis in oligodendrocytes was found only in head injury cases of short survival period in CNPase-stained sections. No changes of micro glia were found in cases of short survival period in RCA1-stained sections.

1.存活期短的病例中星形胶质细胞（AC）的变化：研究了因数例死亡病例中AC的变化，特别是核固缩。对48例因上吊、勒死、溺水、放血等致死的福尔马林固定脑组织进行了检查。从胼胝体的石蜡包埋切片免疫组化染色使用的胶质细胞酸性蛋白（GFAP）的抗体。AC核固缩27例。27例中有25例存活时间少于1小时。其中，所有窒息死亡病例均见核固缩。此外，在因失血死亡的病例中，在所有存活时间少于1小时的病例中均检测到固缩，而在存活时间超过1小时的病例中均未检测到固缩。因此，检测AC核固缩可作为判断存活期的参考指标.在几个切片上发现AC核固缩：在一例因失血致死而存活瓦尔数分钟的病例中，在胼胝体和大脑白色物质切片上发现AC核固缩，而在脑干和小脑的切片上均未发现。在1例枪击致死病例中，胼胝体、大脑白色质、脑干和小脑皮质均可见AC核固缩.胶质细胞变化的观察：CNPases染色切片中，少突胶质细胞核固缩仅见于存活期较短的颅脑损伤病例。存活期短的病例在RCA 1染色切片中未发现小胶质细胞的变化。