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Immunohistochemical studies on early changes of components of the nervous system after head injury and application to forensic diagnosis

颅脑损伤后神经系统组成早期变化的免疫组织化学研究及其在法医学诊断中的应用

基本信息

批准号：
10670395
负责人：
OGATA Mamoru
金额：
$ 1.66万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

项目摘要

1. Immunohistochemical studies on axonal injury after head trauma: Further studies were performed to determine whether immunostaining for neuron-specific enolase (NSE) can detect early post-traumatic axonal changes. NSE as well as amyloid precursor protein (APP) could label so-called axonal bulbs and swollen axons in cases with only 1.5 hours' survival after head trauma, so that NSE should be one of useful markers for detection of early axonal changes.2. Changes of astrocytes after brain injury: Antibodies against glial fibrillary acidic protein (GFAP), S100 protein, vimentin, laminin and glutamine synthetase (GS) were used. Immunostaining for GFAP and S100 labelled both reactive astrocytes in cases of head injury with more than 7 days' survival and swollen astrocytes in cases with 9 hours' to 7 days' survival. Further, in cases with less than 1.5 hours' survival, clasmatodendrosis and pyknosis of the nucleus were detected by GFAP and S100 staining. Immunostaining for vimentin, laminin and GS also labelled these astrocytes, suggesting that immunostaining for astrocytes should be useful for evaluating early brain damage after head injury.3. Changes of oligodendrocytes after brain injury: Immnostaining for CNPase label oligodendrocytes of head trauma, though did not label cells of control brain. Therefore it is suggested that CNPase staining is very useful for detection of early oligodendrocytic changes after head injury.4. Changes of microglia after brain injury: Lectin staining for Ricinus Communis agglutinin-1 (RCA1) labelled so-called foamy cells in cases of head injury with more than 2 weeks' survival and hypertrophic microglia in cases with more than 9 hours' survival, so that lectin staining for RCA1 may be useful for estimating the duration of posttraumatic survival.

1.头部创伤后轴突损伤的免疫组织化学研究：进行进一步研究以确定神经元特异性烯醇化酶（NSE）的免疫染色是否可以检测早期创伤后轴突变化。结论：1. NSE和淀粉样前体蛋白（APP）均可在脑外伤后存活仅1.5小时的病例中标记出所谓的轴突球和肿胀的轴突，因此NSE可作为检测早期轴突改变的有用标志物之一.脑损伤后星形胶质细胞的变化：采用胶质细胞酸性蛋白（GFAP）、S 100蛋白、波形蛋白、层粘连蛋白和谷氨酰胺合成酶（GS）抗体。GFAP和S100的免疫染色既标记了存活超过7天的脑损伤病例中的反应性星形胶质细胞，也标记了存活9小时至7天的病例中的肿胀星形胶质细胞。在存活时间小于1.5小时的病例中，GFAP和S100染色检测到细胞核的分裂和固缩。波形蛋白、层粘连蛋白和GS的免疫染色也标记了这些星形胶质细胞，提示星形胶质细胞的免疫染色对于评估颅脑损伤后的早期脑损伤是有用的.脑损伤后少突胶质细胞的变化：脑损伤组少突胶质细胞经免疫组化染色，未标记对照组。因此，CNB染色对检测颅脑损伤后早期少突胶质细胞的变化非常有用.脑损伤后小胶质细胞的变化：在存活超过2周的脑损伤病例中，凝集素染色显示蓖麻凝集素-1（RCA 1）标记的所谓泡沫细胞，在存活超过9小时的病例中，凝集素染色显示肥大的小胶质细胞，因此RCA 1的凝集素染色可能有助于估计创伤后存活时间。