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Study on differential diagnosis of traumatic or intrinsic cerebral hematoma of the basal nucleus by use of immunohistochemical markers of blood vessels

血管免疫组化标记物鉴别诊断外伤性或内源性基底核血肿的研究

基本信息

批准号：
17590581
负责人：
OGATA Mamoru
金额：
$ 2.24万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

项目摘要

1. Immunohistochemical studies on axonal changes concerning differential diagnosis of intrinsic or traumatic cerebral hematoma : Immunostaining for amyloid precursor protein (APP), neuron-specific enolase (NSE) and neurofilament (NF) 160 labeled varicose axons and axonal bulb in traumatic cases of more than 2 hours-survival. Immunostaining for mitochondria leveled axonal bulb in traumatic cases of more than 1 day-survival. Immunostaining for NF 200 leveled axons regardless of traumatic or non-traumatic cases.2. Immunohistochemical studies on markers of the blood vessels : Immunostaining for angiotensin converting enzyme (ACE) leveled unstable plaques of arteriosclerosis in cases of intrinsic cerebral hematoma. However, ACE labeled plaques in one case of intrinsic hematoma.3. Application to actual autopsy cases : In a case of death 3 weeks after suffering from a traffic accident, an autopsy revealed left extensive cerebral hematoma, cerebral contusion and subarach- noidal hemorrhage. Immunostaining for APP and NSE leveled axonal bulbs. Immunostaining for ACE failed to label vessels. From these findings, we diagnosed that the hematoma is due to trauma. In a case of death after suffering from a lot of skin injuries, an autopsy revealed left extensive cerebral hematoma. Immunostaining for ACE labeled many vessels. From these findings, we judged that the hematoma is due to trauma. In this case, immunostaining for NSE leveled axonal bulbs, suggesting that it should be important to examine several immunostainings before diagnosing.

1.免疫组化研究轴突变化与内源性或创伤性脑血肿的鉴别诊断：淀粉样前体蛋白（APP），神经元特异性烯醇化酶（NSE）和神经丝（NF）160标记的静脉曲张的轴突和轴突球在超过2小时的创伤病例中的免疫染色-存活。存活1天以上的创伤患者轴索球线粒体水平的免疫组化染色。免疫组化染色显示NF 200水平的轴突，无论是创伤性还是非创伤性.血管标记物的免疫组织化学研究：血管紧张素转换酶（ACE）的免疫染色使内源性脑血肿病例中的动脉硬化不稳定斑块变平。但在1例内源性血肿中，ACE标记斑块。应用于实际尸检病例：1例交通事故3周后死亡，尸检发现左侧广泛脑血肿、脑挫伤和蛛网膜下腔出血。APP和NSE水平轴突球的免疫染色。ACE免疫染色未能标记血管。根据这些发现，我们诊断血肿是由于创伤。在一个案例中，死者在遭受了大量的皮肤损伤后，尸检发现留下了广泛的脑血肿。免疫组化显示ACE标记的血管较多。根据这些发现，我们判断血肿是由创伤引起的。在这个病例中，神经元特异性烯醇化酶的免疫染色使轴突球变平，提示在诊断前检查几种免疫染色是很重要的。