Experimental studies of the tumor microvessels using monochromatic synchrotron radiation

单色同步辐射肿瘤微血管的实验研究

• 批准号: 14570888
• 负责人: IMAI Shigeki
• 金额: $ 2.05万
• 依托单位: KAWASAKI MEDICAL SCHOOL
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14570888/
• 关键词: Synchrotron radiation; Monochromatic x-ray; Microangiography; Malignant tumor; Anticancer drug; Angiogenesis inhibitor; 悪性腫瘍; 腫瘍血管

Purpose : To evaluate changes in tumor microvessels after administration of anticancer drugs and angiogenesis inhibitor using synchrotron radiation in vivo.Material and Methods : 1.Anticancer drugs : VX2 carcinomas were transplanted into the auricles of 40 rabbits and three days later the rabbits were divided into two groups ; a group given anticancer drugs by intraarterial injection(IA) and a group given anticancer drugs by drip infusion(DIV). The two groups were administrated Cisplatin, Carboplatin, Docetaxel hydrate and Adriamycin. Five untreated VX2 carcinomas served as control group. 2.Angiogenesis inhibitor : The FR-118487 agent was continuously infused subcutaneously to 30 rabbits with VX2 carcinoma implantation at auricles. The rabbits were divided into six groups : control-3 and control-7 received only propylene glycol for three or seven days, FR-1-3 and FR-1-7 received 1mg/day/kg of FR-118487 for three or seven days, FR-3-3 and FR-3-7 received 3mg/day/kg of FR-118487 for thre … More e or seven days. Microangiograms of VX2 carcinomas were obtained with a synchrotron radiation system.Results : 1.Anticancer drugs : In the control group, the number of tumor microvessels remained stationary. In the IA group, the number of tumor microvessels had decreased after 5 min. In the DIV group, the number of tumor microvessels had decreased after 15 min. Changes in microvessels of approximately 50 to 200μm in diameter. 2.Angiogenesis inhibitor : The control-3 group shows as a hypervascular lesion. In the control-7 group, the hypovascular area on the microangiogram has increased. In the FR group, In the FR-x-3 group, there were a fewer observed irregular vessels than control-3 group. In the FR-x-7group shows many irregular vessels and arteriovenous shunts presented within the tumors. These findings were similar to the control-3 group.Conclusion : The synchrotron radiation system may be a useful tool for changes in tumor microvessels after administration of anticancer drugs and angiogenesis inhibitor. Less

目的：观察同步辐射体内应用抗癌药物和血管生成抑制剂后肿瘤微血管的变化。材料与方法：1.抗癌药物：40只兔耳廓内移植VX2肿瘤，3d后随机分为两组：动脉注射(IA)组和静脉滴注组(DIV)。两组分别给予顺铂、卡铂、多西紫杉醇和阿霉素治疗。5例未经治疗的VX2肿瘤作为对照组。2.血管生成抑制剂：30只兔耳廓种植VX2肿瘤，皮下持续注入FR-118487制剂。将兔分为6组：对照组3和对照组7仅给予丙二醇组，FR-1-3和FR-1-7组给予FR-118487 1 mg/d/kg，连续3或7天，FR-3-3和FR-3-7组给予FR-118487 3 mg/d/kg，用于Thre…再多几天或七天。结果：1.抗癌药物组：对照组肿瘤微血管数量基本不变。IA组5min后肿瘤微血管数量减少。DIV组15min后肿瘤微血管数量减少。直径约50-200μm的微血管的变化。2.血管生成抑制因子：对照-3组表现为多血管病变。在对照-7组，微血管造影上的小血管面积增加。在FR组中，FR-x-3组中观察到的不规则血管较对照-3组少。FR-x-7组肿瘤内可见多条不规则血管和动静脉分流。这些发现与对照3组相似。结论：同步辐射系统可能是一种有用的工具，用于研究给予抗癌药物和血管生成抑制剂后肿瘤微血管的变化。较少

项目成果

Yamashita T et: "Evaluation of the tumor microvessels using Monochromatic X-rays"Tomorrow Synchrotron Clinical Application. 75-83 (2003)

Yamashita T 等人：“使用单色 X 射线评估肿瘤微血管”明天同步加速器临床应用。

T.Yamashita: "Evaluation of tumor microvessels using Monochromatic X-rays"Tomorrow Synchrotron Clinical Application. 75-83 (2003)

T.Yamashita：“使用单色 X 射线评估肿瘤微血管”明天同步加速器临床应用。