权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

H1 Receptor-Expressing Afferent Neurons After Peripheral Axotomy and Naturopathic Pain.

周围轴突切开术和自然疗法疼痛后表达 H1 受体的传入神经元。

基本信息

批准号：
14571480
负责人：
KASHIBA Hitoshi
金额：
$ 2.05万
依托单位：
Kansai Coll.of Oriental Med.
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571480/
关键词：
histamine H1 receptor primary sensory neuron rat guinea pig capsaicin H1アンタゴニスト神経傷害 in situ ハイブリダイゼーション

项目摘要

Pharmacological studies have suggested that a subgroup of primary sensory neurons is responsive to histamine via HI receptor. However, which type of primary sensory neurons express HI receptor is not known. We addressed this issue using in situ hybridization histochemistry with a cRNA probe for the guinea pig H1 receptor mRNA. H1 receptor mRNA was expressed in about 15-20% of the trigeminal and lumbar dorsal root ganglion(DRG) neurons, but none of nodose ganglion neurons. The positive neurons in DRG were exclusively small in size, and were labeled by isolectin B4, suggesting that these neurons have unmyelinated fibers. However, H1 receptor mRNA-expressing DRG neurons were not immunoreaetive to SP or CGRP, which are implicated in the nociceptive transmission of the primary sensory system. Moreover, in guinea pigs neonatally treated with capsaicin(50mg/kg), few CGRP-immunoreaetive neurons were seen in DRG, but the percentage of H1 receptor mRNA-expressing neurons(15%-20%) and the intensity of the mRNA signals in these neurons were not affected by neonatal capsaicin treatment, suggesting that H1 receptor-expressing neurons are not sensitive to capsaicin. These findings suggest that H1 receptor-expressing neurons are involved in the transmission of a unique sensory modality such as itch. A marked increase in the number of mRNA-positive DRG neurons was observed 1-5 days after a crush injury of the sciatic nerve. These neurons turned mRNA-positive after the nerve crush were also mainly small-sized., The mRNA signals were detected in many peptidergic(SP/CGRP) neurons, in contrast to the normal condition. On the other hand, mRNA signals were decreased in the neurons which showed intense labeling in the normal condition. These results suggest that the gene expression of HI receptors up-regulated in injured afferents may be involved in neuropathic pain.

药理学研究表明，初级感觉神经元的一个亚群通过HI受体对组胺产生反应。然而，哪种类型的初级感觉神经元表达HI受体尚不清楚。我们解决了这个问题，使用原位杂交组织化学与cRNA探针的豚鼠H1受体mRNA。H1受体mRNA在三叉神经节和腰脊神经节（DRG）的15-20%的神经元中有表达，而在结状神经节的神经元中无表达。背根节中的阳性神经元尺寸很小，并被同工凝集素B4标记，表明这些神经元具有无髓鞘纤维。然而，表达H1受体mRNA的DRG神经元对SP或CGRP没有免疫反应性，这两种物质与初级感觉系统的伤害性传递有关。另外，在辣椒素（50 mg/kg）处理的豚鼠DRG中，CGRP免疫反应阳性神经元较少，但H1受体mRNA表达神经元的百分比（15%-20%）和mRNA信号强度不受辣椒素处理的影响，提示H1受体表达神经元对辣椒素不敏感。这些发现表明，H1受体表达神经元参与了独特的感觉方式，如瘙痒的传输。坐骨神经挤压伤后1-5天，观察到mRNA阳性DRG神经元数量显著增加。这些在神经挤压后转变为mRNA阳性的神经元也主要是小尺寸的。与正常情况相比，在许多肽能（SP/CGRP）神经元中检测到mRNA信号。另一方面，在正常条件下显示强标记的神经元中，mRNA信号减少。这些结果表明，HI受体的基因表达上调在损伤的传入可能参与神经病理性疼痛。