Characterization of polyamine transport systems and modulation of NMDA receptor by polyamines

多胺转运系统的表征和多胺对 NMDA 受体的调节

• 批准号: 14572052
• 负责人: KASHIWAGI Keiko
• 金额: $ 2.24万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14572052/
• 关键词: Polyamine; Spermine; Cadaverine; Antiporter; Transport; NMDA receptor; ABC transporter; ATPase; プトレスシン; トランスポーター; プロトン駆動力; スペルミジン; 高度好熱菌; イフェンプロジル

1.Cycling of polyamines (spermine and spermidine) in the brain was examined by measuring polyamine transport in synaptic vesicles, synaptosomes and glial cells, and the release of spermine from hippocampal slices. Membrane potential-dependent polyamine transport systems exist in synaptosomes and glial cells, and a proton gradient-dependent polyamine transport system exists in synaptic vesicles. Spermine was found to be accumulated in synaptic vesicles and was released from rat hippocampal slices by depeolarization. Polyamines may function as neuromodulators in the brain.2.Extracellular protons inhibit NMDA receptors. To identify the molecular determinants of proton sensitivity, we used scanning mutagenesis of NR1 subunit. The majority of mutations that affected proton sensitivity were clustered in the extracellular end of the second transmembrane domain and adjacent linker leading to the S2 portion of the glycine binding domain of NR1. The proton sensor and receptor gate may be structu … More rally and functionally integrated.3.The ATPase activity of PotA, a component of the spermidine-preferential uptake system consisting of PotA, B, C, and D, was studied using purified PotA and a PotABC complex. When PotA was associated with PotB and PotC, PotA became more sensitive to inhibition by spermidine, suggesting that spermidine functions as a feedback inhibitor. The NH_2-terminal domain contains the ATP binding pocket andt he active center of ATPase. The COON-terminal domain of PotA contains a site that regulates ATPase activity and a site involved in the spermidine inhibition of ATPase activity.4.The functions of cadaverine transport protein CadB were studied in Escherichia colt: CadB had both cadaverine uptake and cadaverine excretion activities. At acidic pH, the operon containing cadB and cadA, encoding lysine decarboxylase, was induced in the presence of lysine, leading to generation of a proton motive force. The cadBA operon plays important roles in cellular regulation at acidic pH. Less

1.多胺（精胺和亚精胺）在大脑中的循环进行了检查，通过测量多胺运输突触囊泡，突触体和神经胶质细胞，以及从海马切片精胺的释放。突触体和神经胶质细胞中存在膜电位依赖的多胺转运系统，突触囊泡中存在质子梯度依赖的多胺转运系统。精胺在突触囊泡中积累，并通过去极化从大鼠海马脑片中释放。多胺可能在脑内起神经调质的作用。2.细胞外质子抑制NMDA受体。为了确定质子敏感性的分子决定因素，我们使用NR1亚基的扫描诱变。影响质子敏感性的大多数突变聚集在第二跨膜结构域的细胞外末端和相邻接头中，导致NR1的甘氨酸结合结构域的S2部分。质子传感器和受体门可以被构造成 ...更多信息 3.用纯化的PotA和PotABC复合物研究了由PotA、B、C和D组成的亚精胺优先摄取系统的组分PotA的ATP酶活性。当PotA与PotB和PotC结合时，PotA对亚精胺的抑制作用变得更加敏感，这表明亚精胺具有反馈抑制剂的功能。NH_2端结构域含有ATP结合口袋和ATP酶活性中心。PotA的COON末端含有一个调节ATP酶活性的位点和一个参与亚精胺抑制ATP酶活性的位点。4.对大肠杆菌中的尸胺转运蛋白CadB的功能进行了研究：CadB既具有尸胺摄取活性，又具有尸胺排泄活性。在酸性pH下，在赖氨酸的存在下诱导含有cadB和cadA的编码赖氨酸脱羧酶的操纵子，从而产生质子动力。cadBA操纵子在酸性pH下的细胞调节中起重要作用。

项目成果

Low, C.-M.et al.: "Molecular determinants of proton-sensitive N-methyl-D-aspartate receptor gating"Mol.Pharmacol.. 63. 1212-1222 (2003)

Low, C.-M.等人：“质子敏感性 N-甲基-D-天冬氨酸受体门控的分子决定因素”Mol.Pharmacol.. 63. 1212-1222 (2003)

Low, C.-H.et al.: "Molecular determinants of proton-sensitive N-methyl-D-aspartate receptor gating"Mol.Pharmacol.. 63. 1212-1222 (2003)

Low, C.-H.等人：“质子敏感性 N-甲基-D-天冬氨酸受体门控的分子决定因素”Mol.Pharmacol.. 63. 1212-1222 (2003)

Yoshida, M. et al.: "Polyamines enhance synthesis of the RNA polymerase σ^<38> subunit by suppression of an amber termination codon in the open reading frame"J.Biol.Chem.. 277. 37139-37146 (2002)

Yoshida, M. 等人：“多胺通过抑制开放阅读框中的琥珀终止密码子来增强 RNA 聚合酶 σ^<38> 亚基的合成”J.Biol.Chem.. 277. 37139-37146 (2002)

Kashiwagi, K.et al.: "Channel blockers acting N-methyl-D-aspartate receptors : Differential effects of mutations in the vestibule and ion channel pore."Mol.Pharmacol.. 61. 533-545 (2002)

Kashiwagi, K.等人：“作用 N-甲基-D-天冬氨酸受体的通道阻滞剂：前庭和离子通道孔突变的差异效应。”Mol.Pharmacol.. 61. 533-545 (2002)

Kashiwagi, K. et al.: "Channel blockers acting at N-methyl-D-aspartate receptors : Differential effects of mutations in the vestibule and ion channel pore"Mol.Pharmacol.. 61. 533-545 (2002)

Kashiwagi, K.等：“作用于N-甲基-D-天冬氨酸受体的通道阻滞剂：前庭和离子通道孔中突变的差异效应”Mol.Pharmacol.. 61. 533-545 (2002)