Molecular mechanism and physiological role of polyamine transport systems and NMDA receptors

多胺转运系统和NMDA受体的分子机制和生理作用

• 批准号: 18590069
• 负责人: KASHIWAGI Keiko
• 金额: $ 2.57万
• 依托单位: Chiba Institute of Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18590069/
• 关键词: Polyamine; Spermidine; Spermine; polyamine transport; NMDA receptor; Phosphorylation; Antiporter; Channel blocker; ポリアミン輸送; 排出蛋白質; R domain

1. N-Methyl-D-aspartate(NMDA)receptors are involved in synaptic plasticity and may also play a role in seizure activity. Overactivation of these receptors can lead to neuronal cell death. Thus, NMDA receptors are potential targets for anticonvulsants and neuroprotective agents. We found that anthraquinone (AQ)- and anthracene (Ant)-tetraamines are potent NMDA channel blockers. The effects of both AQ- and Ant-tetraamines were reversible and voltage-dependent. Results of experiments using mutant NR1 and NR2B subunits of NMDA receptor identified residues that influence block by AQ- and Ant-tetraamines. The results indicate that the polyamine tail is crucial for block by AQ- and Ant-tetraamines. Residues in the outer vestibule of the NR1 subunit were more strongly involved in block by AQ- and Ant-tetraamines than residues in the corresponding region of NR2B.2. CadB, a cadaverine-lysine antiporter, is strongly involved in cell growth under acidic conditions in Escherichia coll. Amino acid residues involved in the activity of CadB were determined by site-directed mutagenesis of the gene. It was found that Cys370 is essential for the activity and was suggested that a hydrophilic cavity is formed by the transmembrane segments II, III, IV, VI, VII, X, XI and XII.3. We have looked for polyamine-preferential uptake proteins in S. cerevisiae. DUR3 catalyzed the uptake of polyamines together with urea, and SAM3 catalyzed the uptake of polyamines together with S-adenosylmethionine, glutamic acid and lysine. Polyamine uptake was greatly decreased in both DUR3- and SAM3-deficient cells. Polyamine stimulation of cell growth of a polyamine requiring mutant was completely inhibited by the disruption of both DUR3 and SAM3. The results indicate that DUR3 and SAM3 are major polyamine uptake proteins in yeast. It was found that DUR3, but not SAM3, was activated by phosphorylation by polyamine transport protein kinase 2.

1. N-甲基-D-天冬氨酸（NMDA）受体参与突触可塑性，也可能在癫痫发作活动中发挥作用。这些受体的过度激活可导致神经元细胞死亡。因此，NMDA受体是抗惊厥药和神经保护剂的潜在靶点。我们发现蒽醌（AQ）-和蒽（Ant）-四胺是有效的NMDA通道阻滞剂。AQ-和Ant-tetraamine的作用都是可逆的和电压依赖性的。使用NMDA受体的突变体NR 1和NR 2B亚基的实验结果鉴定了影响AQ-和Ant-tetraamine阻断的残基。结果表明，多胺尾部对于AQ-和Ant-tetraamine的封闭是至关重要的。NR 1亚基的外前庭中的残基比NR 2B的相应区域中的残基更强烈地参与AQ-和Ant-tetraamine的阻断。CadB是一种尸胺-赖氨酸反向转运蛋白，在大肠杆菌中酸性条件下强烈参与细胞生长。通过定点突变该基因，确定了参与CadB活性的氨基酸残基。发现Cys 370对于活性是必需的，并且表明由跨膜区段II、III、IV、VI、VII、X、XI和XII.3形成亲水腔。我们在S.啤酒。DUR 3催化多胺与尿素一起的摄取，SAM 3催化多胺与S-腺苷甲硫氨酸、谷氨酸和赖氨酸一起的摄取。在DUR 3和SAM 3缺陷细胞中，多胺摄取大大降低。多胺刺激细胞生长的多胺需要突变体被完全抑制的破坏DUR 3和SAM 3。结果表明，DUR 3和SAM 3是酵母中主要的多胺摄取蛋白。结果发现，DUR 3，而不是SAM 3，被激活的磷酸化多胺转运蛋白激酶2。

项目成果

Docosahexaenoic acid inhibits growth of mouse mammary cancer cells by interfering with p27/kipl, Cdk2, ERK1/ERK2 and retinoblastoma phosphorylation.

二十二碳六烯酸通过干扰 p27/kipl、Cdk2、ERK1/ERK2 和视网膜母细胞瘤磷酸化来抑制小鼠乳腺癌细胞的生长。

• 发表时间: 2006
• 期刊: J.Lipid.Res 47
• 作者: Khan;N;A;et al.
• 通讯作者: et al.

Polyamine Uptake by DUR3 and SAM3 in Saccharomyces cerevisiae*

• DOI: 10.1074/jbc.m611105200
• 发表时间: 2007-03
• 期刊: Journal of Biological Chemistry
• 影响因子: 4.8
• 作者: T. Uemura;K. Kashiwagi;K. Igarashi

Polyamines in renal failure : Review article

肾衰竭中的多胺：评论文章

• 发表时间: 2006
• 期刊: Amino Acids 31
• 作者: Igarashi;K;et al.
• 通讯作者: et al.

Decrease in polyamines with aging and their ingestion from food and drink

• DOI: 10.1093/jb/mvj003
• 发表时间: 2006-01-01
• 期刊: JOURNAL OF BIOCHEMISTRY
• 影响因子: 2.7
• 作者: Nishimura, K;Shiina, R;Igarashi, K
• 通讯作者: Igarashi, K

Anthraquinone polyamines : novel channel blockers on N-methyl-D-aspartate receptors

蒽醌多胺：N-甲基-D-天冬氨酸受体的新型通道阻滞剂

• 发表时间: 2007
• 期刊: Amino Acids 33
• 作者: Kashiwagi;K.;et. al.
• 通讯作者: et. al.