Physiological role of polyamines and regulation of their cellular contents

多胺的生理作用及其细胞内容物的调节

• 批准号: 23590088
• 负责人: KASHIWAGI Keiko
• 金额: $ 3.41万
• 依托单位: Chiba Institute of Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590088/
• 关键词: 生理活性アミン; ポリアミン; アクロレイン; 脳・神経; NMDA受容体; プトレスシン輸送; スペルミジン; スペルミン; プトレスシン; グルタチオン; GAPDH; バイオフィルム; 生存率; ppGpp

Polyamines (putrescine, spermidine and spermine) play important roles in cell proliferation and differentiation. The physiological role of polyamines and regulation of their cellular contents were examined. 1) Five genes were identified as members of "polyamine modulon", whose expression is enhanced by polyamines at the level of translation, and were involved in maintenance of cell viability in Escherichia coli. 2) For elucidation of the role of polyamines on neural functions, spermine and ifenprodil binding sites on R domains of NMDA receptors were identified and the structural change of R domains by binding of these compounds were elucidated. Acrolein, a polyamine metabolite, was found to be involved in neurodegeneration after stroke. Mechanisms of cell toxicity of acrolein were examined. 4) Physiological role of two putrescine transport systems were clarified in E. coli.

多胺(腐胺、亚精胺和精胺)在细胞增殖和分化中起重要作用。研究了多胺的生理作用及其对细胞含量的调节。1)5个基因被鉴定为“多胺调节子”成员，它们在翻译水平上被多胺增强，并参与维持大肠杆菌的细胞活力。2)为了阐明多胺对神经功能的影响，我们鉴定了NMDA受体R结构域上的精胺和异丙苯地尔的结合部位，并阐明了这些化合物结合后R结构域的结构变化。丙烯醛是一种多胺代谢产物，被发现与中风后的神经退行性变有关。探讨了丙烯醛的细胞毒性机制。4)阐明了两种腐胺转运系统在大肠杆菌中的生理作用。

项目成果

Role of polyamines at the G1/S boundary and G2/M phase of the cell cycle.

• DOI: 10.1016/j.biocel.2013.02.021
• 发表时间: 2013-06
• 期刊: The international journal of biochemistry & cell biology
• 作者: Tomoko Yamashita;K. Nishimura;Ryotaro Saiki;H. Okudaira;M. Tome;Kyohei Higashi;Mizuho Nakamura;Y. Terui;K. Fujiwara;K. Kashiwagi;K. Igarashi

アクロレイン抱合GAPDHを介したアクロレインの細胞毒性機序

丙烯醛通过丙烯醛缀合的 GAPDH 的细胞毒性机制

• 作者: 中村瑞穂;富取秀行;鈴木健裕;照井祐介;斎木遼太郎;堂前 直;五十嵐一衛;柏木敬子
• 通讯作者: 柏木敬子

Enhanced cell viability by polyamines under oxidative stress through polyamine stimulation of SoxR and EmrR in Escherichia coli.

通过多胺刺激大肠杆菌中的 SoxR 和 EmrR，多胺在氧化应激下增强细胞活力。

• 发表时间: 2013
• 作者: Terui;Y.;Sakamoto;A.;Tamada;A.;Ichihara;T.;Yamamoto;T.;Yamamoto;K.;Ishihama;A.;Igarashi;K.;Kashiwagi;K.
• 通讯作者: K.

マウス神経芽細胞腫及びマウス乳がん細胞におけるアクロレイン毒性の解毒機構

丙烯醛毒性对小鼠神经母细胞瘤和小鼠乳腺癌细胞的解毒机制

• 发表时间: 2012
• 作者: 富取秀行;中村瑞穂;坂本明彦;照井祐介;吉田円;柏木敬子;五十嵐一衛
• 通讯作者: 五十嵐一衛

Enhanced biofilm formation and cell viability by polyamines through polyamine stimulation of response regulators UvrY and CpxR in the two-component signal transducing systems and ribosome recycling factor.

通过多胺刺激双组分信号转导系统和核糖体循环因子中的反应调节剂 UvrY 和 CpxR，增强生物膜形成和细胞活力。

• 发表时间: 2012
• 作者: Sakamoto;A.;Terui;Y.;Yamamoto;T.;Kasahara;T.;Tomitori;H.;Yamamoto;K.;Ishihama;A.;Michael;A. J.;Igarashi;K.;Kashiwagi;K.
• 通讯作者: K.