Studies on the Mechanism of Cell Deterioration for the Prevention of Aging through Dietary Life

通过饮食生活预防衰老的细胞退化机制研究

• 批准号: 16500526
• 负责人: DOI Hiroshi
• 金额: $ 2.37万
• 依托单位: Mukogawa Women's University (2004, 2006)Mukogawa Women's University Junior College Division (2005)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16500526/
• 关键词: oxidative stress; phosphatidylcholine hydroperoxide; membrane lipid; microtubules; tubulin; PC12細胞; 細胞膜損傷; 抗酸化系; GTPase; 細胞膜; 細胞分化; 神経突起; 細胞骨格; 細胞生育; 神経突起形成

Lipid peroxidation traditionally has been regarded as the major process causing damage to the brain by oxygen radicals and, subsequently, some forms of age-related deterioration. The brain, with its high oxygen consumption and abundance of polyunsaturated fatty acids, is at risk for oxidative damage. Although a great number of reports have associated lipid peroxidation with neurodegenerative diseases, the precise target of lipid peroxidation remains unclear. The aim of this study was to clarify the precise target of lipid hydroperoxides in neuronal cells. In this study, we showed the deleterious effect of phosphatidylcholine hydroperoxides (PCOOH) on PC 12 cells before and after differentiation into neuronal cells. Cell viability was significantly decreased in differentiated cells treated with PCOOH compared to undifferentiated cells treated in a similar way. PCOOH disrupted the formation of neurites and neuronal microtubules, which consist mainly of tubulin. Our results showed that differentiated cells were more vulnerable than undifferentiated cells to PCOOH and that PCOOH could attack microtubule-tubulin systems. This is the first study to clarify the effect on neuronal cells of PCOOH produced in the early stage of neurodegenerative disease and to elucidate the target of lipid hydroperoxide.

脂质过氧化传统上被认为是氧自由基对大脑造成损伤的主要过程，并随后导致某些形式的与年龄相关的退化。大脑因其高耗氧量和丰富的多不饱和脂肪酸，有氧化损伤的风险。尽管大量的报道将脂质过氧化与神经退行性疾病联系起来，但脂质过氧化的确切目标仍不清楚。本研究的目的是阐明脂质氢过氧化物在神经元细胞中的精确靶点。在本研究中，我们展示了磷脂酰胆碱氢过氧化物（PCOOH）对pc12细胞分化为神经元细胞前后的有害作用。与未分化细胞相比，经PCOOH处理的分化细胞的细胞活力显著降低。PCOOH破坏主要由微管蛋白组成的神经突和神经元微管的形成。我们的研究结果表明，分化的细胞比未分化的细胞更容易受到PCOOH的攻击，PCOOH可以攻击微管-微管蛋白系统。本研究首次阐明了神经退行性疾病早期产生的PCOOH对神经元细胞的影响，并阐明了脂质过氧化氢的作用靶点。

项目成果

日本産大麦分級粉からの微小管形成促進物質の精製

日本大麦粉中微管形成促进物质的纯化

• 发表时间: 2007
• 期刊: 食品・臨床栄養 2・1
• 作者: 川口真規子;関口綾子;山中裕佳子;土井裕司
• 通讯作者: 土井裕司

Sustrate specificity of aminopeptidase from Japanese classified barley flour

日本分级大麦粉氨肽酶的底物特异性

• 发表时间: 2006
• 期刊: Journal of Food Biochemistry 30・2
• 作者: 川口真規子;土井裕司
• 通讯作者: 土井裕司

Effects on immune response of antidiabetic ingredients from white-skinned sweet potato (Jpomoea batatas L.)

白皮甘薯 (Jpomoea batatas L.) 抗糖尿病成分对免疫反应的影响

• 发表时间: 2005
• 期刊: Nutrition 21-3
• 作者: Miyazaki;Yoshiko;Kusano;Shuichi;Doi;Hiroshi;Aki;Osami
• 通讯作者: Osami

Purification of microtubule assembly promoting material from Japanese classified barley flour

日本分级大麦粉中微管组装促进物质的纯化

• 发表时间: 2007
• 期刊: Food and Clinical Nutrition 2-1
• 作者: Kawaguchi;Makiko;Sekiguchi;Ayako;Yamanaka;Yukako;Doi;Hiroshi
• 通讯作者: Hiroshi

Sustrate specificity of aminopeptidase from Japanese classified barley flour.

日本分级大麦粉氨肽酶的基质特异性。

• 发表时间: 2006
• 期刊: Journal of Food Biochemistry 30-2
• 作者: Kawaguchi;Makiko;Doi;Hiroshi
• 通讯作者: Hiroshi