Development of foodstuffs for the prevention of ageing based on the cell deterioration mechanism

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In this study, the cell deterioration mechanism by phospholipid hydroperoxides was examined first, and the effect of some food constituents for the deterioration was shown. PC12 cell line was initially derived from rat adrenal phenocromocytoma. PV12 cells differentiate into sympathetic neuron-like phenotypes in response to the nerve growth factor. PC 12 cell were cultured in collagen-coated dishes in RPMI-1640 medium supplemented with 10% heat-inactivated horse serum, 5% heat-inactivated fetal bovine serum and penicillin/streptomycin in the presence of adequate amounts of phospholipid hydroperoxides. Phospholipid hydroperoxides oxidized cell membrane and next deteriorated tubulin by the interaction. The effects on antioxidant enzyme activities and the oxidative damage of DNA were observed also. The interaction between phospholipid hydroperoxides and tubulin introduced the decrease of microtubule assembly, and the functions of microtubule were deteriorated. Dosed and derived reactive oxygen species influenced antioxidant enzyme activities and DNA. Therefore, the recovery effects by antioxidants from foodstuffs were examined. Antioxidants made functions tubulin and antioxidant enzyme to recover. The facts indicate antioxidants effective on the ageing by reactive oxygen species.