Production of wine with high resveratrol content using non-vinifera grapes

白藜芦醇是众所周知的积累在葡萄皮和种子在应对环境压力，如真菌感染。白藜芦醇是一种具有显著生物功能活性的多酚，包括抗癌活性。在本研究中，我们的目的是生产高含量的白藜芦醇葡萄酒暴露于UV-C，一种环境胁迫，诱导reveratrol的生产. UV-C辐射诱导葡萄产生白藜芦醇用UV-C（波长：253.7nm）以10 cm的距离照射葡萄。辐照10- 20 min诱导效果最好，此时葡萄皮中白藜芦醇含量可达0.5mg/g鲜重.滤膜对葡萄酒中白藜芦醇测定的影响研究了白藜芦醇在滤膜上的吸附。醋酸纤维素膜对白藜芦醇有较强的吸附作用，而聚四氟乙烯和氧化铝膜对白藜芦醇的吸附作用不明显.还原剂对白藜芦醇含量的影响白藜芦醇在葡萄汁和葡萄酒中易被氧化。在所使用的还原剂中，亚硫酸盐（焦亚硫酸钾）最能防止白藜芦醇的氧化.光照对酒瓶中白藜芦醇异构化的影响已知白藜芦醇在葡萄中以陷阱形式合成。然而，在葡萄酒中检测到相当数量的顺式白藜芦醇。为了确定葡萄酒生产过程中白藜芦醇异构化的原因，研究了光照的影响。白藜芦醇在白色透明瓶中，在荧光灯照射下容易异构化。

Resveratrol is known to accumulate in grape skin and seed in response to environmental stress such as fungal infection. Resveratrol is a polyphenol having noteworthy bio-functional activities, including anti-cancer activity. In this research, we aim to produce wines with high resveratrol content by exposing grapes to UV-C, a kind of environmental stress, to induce reveratrol production.1. Induction of resveratrol production in grapes by UV-C irradiationGrapes were irradiated with UV-C (wavelength : 253.7nm) at a distance of 10cm. Maximum induction was achieved by irradiation for 10-20min, and resveratrol content in grape skin was increased to 0.5mg/g fresh weight by this treatment.2. Effect of filter membrane on measurement of resveratrol in wineThe absorption of resveratrol to filter membrane was investigated. Cellulose acetate membrane strongly absorbed resveratrol, whereas PTFE and aluminum oxide membrane did not.3. Effect of reducing agent on resveratrol contentResveratrol was easily oxidized in grape juice and wine. Among the reducing agents used, sulfite (potassium disulfite) best prevented the oxidation ofresveratrol.4. Effect of light on isomerization of resveratrol in wine bottleIt is known that resveratrol is synthesized in the traps form in grape. However, a considerable amount of cis-resveratrol was detected in grape wine. To determine the cause of isomerization of resveratrol during wine production, the effect of light was investigated. Resveratrol in a white-clear bottle was isomerized easily upon fluorescent light irradiation.